Park, Jin Woo Kim, YoungSoo Lee, Kee-Jung Kim, Dong Jin 2024-01-20T15:05:48Z 2024-01-20T15:05:48Z 2021-09-05 2012-03 1043-1802 https://pubs.kist.re.kr/handle/201004/129480 Novel vinylsulfone cyanine dyes (em. 550-850 nm) were designed and synthesized for fluorescent labeling of biomolecules via 1,2-addition reaction in aqueous conditions. Due to the virtue of chemical structures of both fluorophore and reactive group, these dyes could be significantly stable and reactive in various aqueous/organic conditions. A wide variety of pH, temperature, buffer concentration, and protein were tested for the optimal labeling condition. English AMER CHEMICAL SOC N-HYDROXYSUCCINIMIDE ESTERS REAGENTS KINETICS PROBES DNA Novel Cyanine Dyes with Vinylsulfone Group for Labeling Biomolecules Article 10.1021/bc200232d 1 BIOCONJUGATE CHEMISTRY, v.23, no.3, pp.350 - 362 BIOCONJUGATE CHEMISTRY 23 3 350 362 scie scopus 000301700200006 2-s2.0-84863390893 Biochemical Research Methods Biochemistry & Molecular Biology Chemistry, Multidisciplinary Chemistry, Organic Biochemistry & Molecular Biology Chemistry Article N-HYDROXYSUCCINIMIDE ESTERS REAGENTS KINETICS PROBES DNA 형광물질 염료 fluorescence vinylsulfone