Kim, Young-Joo Kim, Han-Cheon Ko, Hyeonseok Amor, Evangeline C. Lee, Jong Wha Yang, Hyun Ok 2024-01-20T15:30:15Z 2024-01-20T15:30:15Z 2021-09-04 2012-03 0278-6915 https://pubs.kist.re.kr/handle/201004/129506 Aurentiacin is a chalcone isolated from Syzygium samarangense. In the present study, we examined the anti-inflammatory effects of aurentiacin in lipopolysaccharide (LPS)-stimulated mouse macrophages. Aurentiacin significantly inhibited LPS-induced nitric oxide (NO) production in RAW264.7 cells concomitantly with the suppression of inducible nitric oxide synthase (iNOS) expression. Aurentiacin also reduced the mRNA levels of pro-inflammatory cytokines such as tumor necrosis factor-a (TNF-alpha) and interleukin-6 (IL-6). Electrophoretic mobility shift assays (EMSAs) and reporter gene assays indicated that DNA binding and transcriptional activities of nuclear factor-kappa B (NF-kappa B)/p65 were decreased by aurentiacin in LPS-stimulated RAW264.7 cells. Moreover, results from chromatin immunoprecipitation (ChIP) assays over the promoter region of the iNOS gene were in agreement with the EMSA results. Pretreatment with aurentiacin prevented the nuclear translocation of p65 by blocking the phosphorylation of I-kappa B kinase (IKK). Aurentiacin also attenuated the phosphorylation (Ser536) and acetylation (Lys310) of p65 and phosphorylation of MAPKs. In an inflammatory animal model, the intraperitoneal (i.p.) injection of aurentiacin suppressed the release of pro-inflammatory cytokines. Moreover, the level of iNOS protein ex vivo was decreased by aurentiacin similar to the result in vitro. Taken together, these results suggest that aurentiacin shows anti-inflammatory activity related to the inhibition of NF-kappa B activation. (C) 2011 Elsevier Ltd. All rights reserved. English PERGAMON-ELSEVIER SCIENCE LTD NF-KAPPA-B NITRIC-OXIDE SYNTHASE ACTIVATED PROTEIN-KINASE TUMOR-NECROSIS-FACTOR INOS EXPRESSION PHOSPHORYLATION INDUCTION CELLS P38 TRANSCRIPTION Inhibitory effects of aurentiacin from Syzygium samarangense on lipopolysaccharide-induced inflammatory response in mouse macrophages Article 10.1016/j.fct.2011.11.050 1 FOOD AND CHEMICAL TOXICOLOGY, v.50, no.3-4, pp.1027 - 1035 FOOD AND CHEMICAL TOXICOLOGY 50 3-4 1027 1035 scie scopus 000303284600083 2-s2.0-84862832203 Food Science & Technology Toxicology Food Science & Technology Toxicology Article NF-KAPPA-B NITRIC-OXIDE SYNTHASE ACTIVATED PROTEIN-KINASE TUMOR-NECROSIS-FACTOR INOS EXPRESSION PHOSPHORYLATION INDUCTION CELLS P38 TRANSCRIPTION Aurentiacin NO iNOS NF-kappa B MAPK