Song, M.-K. Yoon, J.-S. Song, M. Choi, H.-S. Shin, C.-Y. Kim, Y.-J. Ryu, W.-I. Lee, H.-S. Ryu, J.-C. 2024-01-20T15:30:47Z 2024-01-20T15:30:47Z 2021-08-31 2012-03 2005-9752 https://pubs.kist.re.kr/handle/201004/129518 Benzo[a]pyrene (BaP) is a polycyclic aromatic hydrocarbon (PAH) that is carcinogenic to humans. Although the environmental distribution and metabolism of BaP have been reported and many researchers are performing risk-assessment and toxicological studies of BaP by means of physical and chemical measurements, only a few studies have examined the expression of mRNAs and their functions in BaP-induced toxicity. Toxicogenomic technology, a new paradigm in toxicity screening, is a useful approach for evaluating the toxic properties of environmental pollutants. We analyzed gene expression profiles using human oligonucleotide chips and identified genes in human hepatocellular carcinoma (HepG2) cells whose expression changed > 1. 5-fold after exposure to BaP. The expression of 4,048 and 3,926 genes was up-and down-regulated > 1. 5-fold (P < 0. 01), respectively, after exposure. Gene ontology (GO) analysis of these genes revealed significant enrichment in several key biological processes related to DNA damage, including DNA repair, cell cycle arrest, and apoptosis. We also performed a contrastive study of cellular effects in HepG2 cells exposed to BaP, and identified increased expression of related genes, cell cycle arrest, and apoptotic cells. These results suggest that genetic markers of BaP-induced toxicity may be molecular blueprints that can be more widely implemented in combination with more traditional techniques for assessment and prediction. ？ 2012 Korean Society of Environmental Risk Assessment and Health Science and Springer. English Kluwer Academic Publishers benzo[a]pyrene messenger RNA polycyclic aromatic hydrocarbon apoptosis article cell culture cell cycle arrest cell proliferation cell strain HepG2 cell viability controlled study cytotoxicity DNA damage DNA hybridization DNA microarray DNA repair environmental exposure flow cytometry gene expression genetic analysis human human cell liver cell carcinoma nucleotide sequence pollution real time polymerase chain reaction reverse transcription polymerase chain reaction risk assessment RNA extraction toxicity toxicity testing unindexed sequence Gene expression analysis identifies DNA damage-related markers of benzo[a]pyrene exposure in HepG2 human hepatocytes Article 10.1007/s13530-012-0118-5 1 Toxicology and Environmental Health Sciences, v.4, no.1, pp.19 - 29 Toxicology and Environmental Health Sciences 4 1 19 29 scopus 2-s2.0-84872298607 Article benzo[a]pyrene messenger RNA polycyclic aromatic hydrocarbon apoptosis article cell culture cell cycle arrest cell proliferation cell strain HepG2 cell viability controlled study cytotoxicity DNA damage DNA hybridization DNA microarray DNA repair environmental exposure flow cytometry gene expression genetic analysis human human cell liver cell carcinoma nucleotide sequence pollution real time polymerase chain reaction reverse transcription polymerase chain reaction risk assessment RNA extraction toxicity toxicity testing unindexed sequence Apoptosis Benzo[a]pyrene (BaP) Cell cycle arrest DNA damage Gene ontology (GO) Microarray Polycyclic Aromatic Hydrocarbons (PAHs)