Bae, Deok Sung Kim, Young Hoon Pan, Cheol-Ho Nho, Chu Won Samdan, Javzan Yansan, Jamyansan Lee, Jae Kwon 2024-01-20T15:30:49Z 2024-01-20T15:30:49Z 2021-09-04 2012-02-29 1976-6696 https://pubs.kist.re.kr/handle/201004/129520 Protopine is an isoquinoline alkaloid contained in plants in northeast Asia. In this study, we investigated whether protopine derived from Hypecoum erectum L could suppress lipopolysaccharide (LPS)-induced inflammatory responses in murine macrophages (Raw 264.7 cells). Protopine was found to reduce nitric oxide (NO), cyclooxygenase-2 (COX-2), and prostaglandin E-2 (PGE(2)) production by U'S-stimulated Raw 264.7 cells, without a cytotoxic effect. Pm-treatment of Raw 264.7 cells with protopine reduced the production of pro-inflammatory cytokines. These inhibitory effects were caused by blocking phosphorylation of mitogen-activated protein kinases (MAP kinases) and also blocking activation of a nuclear factor kappa-light-chain-enhancer of activated B cells (NF-kappa B). [BMB reports 2012; 45(2): 108-113] English KOREAN SOCIETY BIOCHEMISTRY & MOLECULAR BIOLOGY NITRIC-OXIDE NUCLEAR-FACTOR PHORBOL ESTER KAPPA-B EXPRESSION CYTOKINES INTERLEUKIN-1 INHIBITION ALPHA GENE Protopine reduces the inflammatory activity of lipopolysaccharide-stimulated murine macrophages Article 10.5483/BMBRep.2012.45.2.108 1 BMB REPORTS, v.45, no.2, pp.108 - 113 BMB REPORTS 45 2 108 113 scie scopus kci ART001633824 000301080800008 2-s2.0-84863289787 Biochemistry & Molecular Biology Biochemistry & Molecular Biology Article NITRIC-OXIDE NUCLEAR-FACTOR PHORBOL ESTER KAPPA-B EXPRESSION CYTOKINES INTERLEUKIN-1 INHIBITION ALPHA GENE Cyclooxygenase-2 Cytokine Inflammation Macrophage Nitric oxide Protopine