Arora, Arun Trung Quan Luong Krueger, Matthias Kim, Young Jun Nam, Chang-Hoon Manz, Andreas Havenith, Martina 2024-01-20T15:31:36Z 2024-01-20T15:31:36Z 2022-01-25 2012-02 0003-2654 https://pubs.kist.re.kr/handle/201004/129557 In this work we present a label free quantitative detection method for DNA samples amplified by polymerase chain reaction (PCR) in aqueous medium using terahertz-time domain spectroscopy (THz-TDS) in the frequency range from 0.3 to 1.2 THz. The DNA samples of 133 and 697 base pairs were prepared using PCR. We measured the absorption coefficients of DNA solutions in the concentration range of 0-0.3 ng mu l(-1). For both DNA types, the absorption coefficients decreased with increasing DNA concentrations. The average change in absorption coefficients compared to buffer within the frequency range of 0.8-1.0 THz showed a linear behavior. Our results demonstrate that THz-TDS can detect PCR amplified DNA in aqueous solution with a minimum concentration of 0.1 ng mu l(-1) and a minimum sample volume of 10 mu l. English Royal Society of Chemistry Terahertz-time domain spectroscopy for the detection of PCR amplified DNA in aqueous solution Article 10.1039/c2an15820e 1 Analyst, v.137, no.3, pp.575 - 579 Analyst 137 3 575 579 N scie scopus 000298969000006 Chemistry, Analytical Chemistry Article LABEL-FREE DETECTION SOLVATION DYNAMICS LIQUID WATER HYBRIDIZATION ABSORPTION SEQUENCES HYDRATION THZ QUANTIFICATION AMPLIFICATION