Cho, Hyun-Jong Yoon, In-Soo Yoon, Hong Yeol Koo, Heebeom Jin, Yu-Jin Ko, Seung-Hak Shim, Jae-Seong Kim, Kwangmeyung Kwon, Ick Chan Kim, Dae-Duk 2024-01-20T15:32:24Z 2024-01-20T15:32:24Z 2021-09-05 2012-02 0142-9612 https://pubs.kist.re.kr/handle/201004/129597 Polyethylene glycol (PEG)-conjugated hyaluronic acid-ceramide (HACE) was synthesized for the preparation of doxorubicin (DOX)-loaded HACE-PEG-based nanoparticles, 160 nm in mean diameter with a negative surface charge. Greater uptake of DOX from these HACE-PEG-based nanoparticles was observed in the CD44 receptor highly expressed SCC7 cell line, compared to results from the CD44-negative cell line, NIH3T3. A strong fluorescent signal was detected in the tumor region upon intravenous injection of cyanine 5.5-labeled nanoparticles into the SCC7 tumor xenograft mice; the extended circulation time of the HACE-PEG-based nanoparticle was also observed. Pharmacokinetic study in rats showed a 73.0% reduction of the in vivo clearance of DOX compared to the control group. The antitumor efficacy of the DOX-loaded HACE-PEG-based nanoparticles was also verified in a tumor xenograft mouse model. DOX was efficiently delivered to the tumor site by active targeting via HA and CD44 receptor interaction and by passive targeting due to its small mean diameter (<200 nm). Moreover, PEGylation resulted in prolonged nanoparticle circulation and reduced DOX clearance rate in an in vivo model. These results therefore indicate that PEGylated HACE nanoparticles represent a promising anticancer drug delivery system for cancer diagnosis and therapy. (C) 2011 Elsevier Ltd. All rights reserved. English ELSEVIER SCI LTD DRUG-DELIVERY TISSUE DISTRIBUTION CANCER MICELLES THERAPY CARRIER CD44 Polyethylene glycol-conjugated hyaluronic acid-ceramide self-assembled nanoparticles for targeted delivery of doxorubicin Article 10.1016/j.biomaterials.2011.10.064 1 BIOMATERIALS, v.33, no.4, pp.1190 - 1200 BIOMATERIALS 33 4 1190 1200 scie scopus 000298273400020 2-s2.0-81355138967 Engineering, Biomedical Materials Science, Biomaterials Engineering Materials Science Article DRUG-DELIVERY TISSUE DISTRIBUTION CANCER MICELLES THERAPY CARRIER CD44 Doxorubicin Hyaluronic acid-ceramide-polyethylene glycol Prolonged circulation Theranostic nanoparticle Tumor-targeting