Kim, Sang Min Kang, Suk Woo Jeon, Je-Seung Jung, Yu-Jin Kim, Chul Young Pan, Cheol Ho Um, Byung-Hun 2024-01-20T15:32:49Z 2024-01-20T15:32:49Z 2021-09-04 2012-02 0269-3879 https://pubs.kist.re.kr/handle/201004/129619 Gradient HPLC coupled to Diode Array Detector (DAD), MS/MS and NMR was applied to the rapid structure determination of major compounds of methanol extracts from leaves and roots of Petasites japonicus. The relative antioxidant capacities of the compounds were evaluated by an HPLC system with post-column on-line antioxidant detection based on 2,2'-azinobis-3-ethylbenzothiazoline-6-sulfonic acid radical scavenging. Six compounds were successfully separated on a reverse-phase C18 column and were identified as 5-caffeoylquinic acid (5-CQA), fukinolic acid (FA), 3,5-di-O-caffeoylquinic acid (3,5-DCQA), quercetin-3-O-(6-acetyl)-beta-glucopyranoside (QAG), 4,5-di-O-caffeoylquinic acid (4,5-DCQA) and kaempferol-3-O-(6-acetyl)-beta-glucopyranoside (KAG) by MS/MS and 1H NMR data. Among these compounds, those containing a caffeoyl moiety (5-CQA, FA, 3,5- and 4,5-DCQA) showed relatively strong radical scavenging capacity, with 3,5-DCQA having the greatest radical scavenging capacity in leaf (23.09% of total antioxidant capacity) and root (26.47%) extracts. The relative radical scavenging portion of QAG was only 3.41% in the leaves and KAG did not show any radical scavenging activity. These results demonstrate that the hyphenated HPLC techniques can be successfully applied to rapidly identify structures and evaluate antioxidant activities without prior purification of compounds from plant tissues of P. japonicus. Copyright (C) 2011 John Wiley & Sons, Ltd. English WILEY MAGNETIC-RESONANCE-SPECTROSCOPY LIQUID-CHROMATOGRAPHY LC-NMR NATURAL-PRODUCTS FUKINOLIC ACID ONLINE CONSTITUENTS MS/MS CAPACITY ASSAYS Rapid identification and evaluation of antioxidant compounds from extracts of Petasites japonicus by hyphenated-HPLC techniques Article 10.1002/bmc.1646 1 BIOMEDICAL CHROMATOGRAPHY, v.26, no.2, pp.199 - 207 BIOMEDICAL CHROMATOGRAPHY 26 2 199 207 scie scopus 000298840300010 2-s2.0-84855445999 Biochemical Research Methods Biochemistry & Molecular Biology Chemistry, Analytical Pharmacology & Pharmacy Biochemistry & Molecular Biology Chemistry Pharmacology & Pharmacy Article MAGNETIC-RESONANCE-SPECTROSCOPY LIQUID-CHROMATOGRAPHY LC-NMR NATURAL-PRODUCTS FUKINOLIC ACID ONLINE CONSTITUENTS MS/MS CAPACITY ASSAYS hyphenated techniques LC UV-MS LC-NMR on-line antioxidant activity Petasites japonicus radical scavenging activity