Jung, Ji-Han Kim, Hye-Jung Yeom, Jeonghun Yoo, Changyoung Shin, Jihye Yoo, Jinyoung Kang, Chang Suk Lee, Cheolju 2024-01-20T15:33:55Z 2024-01-20T15:33:55Z 2021-09-05 2012-01 0944-1174 https://pubs.kist.re.kr/handle/201004/129670 Lymph node metastasis (LNM) is recognized as an important factor in the progression of tumor malignancy. It is required to discover molecular markers for the prediction of LNM in gastric cancers (GCs). An isotope coded affinity tag (ICAT) method and mass spectrometry were used for the quantitative profiling of LNM-related proteins. Western blot analysis of the identified proteins and immunohistochemistry on a tissue microarray comprising 120 GC cases were performed for validation. We identified 151 differentially expressed proteins (DEPs) with an abundance ratio greater than 1.5-fold. The proteins upregulated in LNM-negative GCs were largely populated with proteins related to cell death. Among the DEPs, galectin-2 was further tested because its expression level was significantly higher in LNM-negative GCs (similar to 12-fold, p < 0.0001) and its expression is known to be not ubiquitous but confined to the gastrointestinal tract. Immunohistochemical analysis revealed that low expression of galectin-2 was significantly associated with LNM (p = 0.031) and advanced clinical stage (p = 0.024). The association of low galectin-2 with LNM was found even in early GCs (p = 0.020). Our results show that proteomic analysis coupled with immunohistochemistry using tissue microarray is a useful tool for identifying LNM-associated proteins in GC. Also, loss of galectin-2 might play an important role in the aggressiveness of GC. English SPRINGER TOKYO TUMOR-NECROSIS-FACTOR PROTEOME ANALYSIS DIFFERENTIAL EXPRESSION COLORECTAL-CANCER FACTOR-ALPHA APOPTOSIS IDENTIFICATION PROGRESSION BREAST CELLS Lowered expression of galectin-2 is associated with lymph node metastasis in gastric cancer Article 10.1007/s00535-011-0463-1 1 JOURNAL OF GASTROENTEROLOGY, v.47, no.1, pp.37 - 48 JOURNAL OF GASTROENTEROLOGY 47 1 37 48 scie scopus 000300291900005 2-s2.0-84863116117 Gastroenterology & Hepatology Gastroenterology & Hepatology Article TUMOR-NECROSIS-FACTOR PROTEOME ANALYSIS DIFFERENTIAL EXPRESSION COLORECTAL-CANCER FACTOR-ALPHA APOPTOSIS IDENTIFICATION PROGRESSION BREAST CELLS Galectin-2 Gastric cancer ICAT Mass spectrometry Immunohistochemistry