Heo, Dong Nyoung Yang, Dae Hyeok Moon, Ho-Jin Lee, Jung Bok Bae, Min Soo Lee, Sang Cheon Lee, Won Jun Sun, In-Cheol Kwon, Il Keun 2024-01-20T15:34:16Z 2024-01-20T15:34:16Z 2021-09-05 2012-01 0142-9612 https://pubs.kist.re.kr/handle/201004/129688 We describe in this study whether the gold nanoparticle (AuNP) surface-functionalized with PEG, biotin, paclitaxel (PTX) and rhodamine B linked beta-cyclodextrin (beta-CD) (AuNP-5') can be useful as a theranostic agent for cancer therapy without the cytotoxic effect on normal cells. Prior to surface-functionalizing AuNPs, the cytotoxicity of the nanoparticles was evaluated, followed by their cytocompatibility. PTX, an anti-cancer agent, formed inclusion complexations with beta-CD conjugated AuNPs, and effectively released from the AuNP-2' surface-functionalized with PEG, beta-cyclodextrin (beta-CD) and paclitaxel (PTX) using the intracellular glutathione (GSH) level (10 mM). Two types of AuNP-4 surface-functionalized with PEG and rhodamine B linked beta-CD and AuNP-5 surface-functionalized PEG, biotin and rhodamine B linked beta-CD were used for evaluating their specific interaction on cancer cells such as HeLa, A549 and MG63. These were also tested against normal NIH3T3 cell, determining that the AuNP-5 was more effectively involved with the cancer cells. Confocal laser scanning microscopy (CLSM), fluorescence-activated cell-sorting (FACS) and cell viability analyses showed that the AuNP-5' plays a significant role in the diagnosis and therapy of the cancer cells, and may be used in theranostic agents. (C) 2011 Elsevier Ltd. All rights reserved. English ELSEVIER SCI LTD TARGETED DELIVERY CELLS ACID PEPTIDE TAXOL Gold nanoparticles surface-functionalized with paclitaxel drug and biotin receptor as theranostic agents for cancer therapy Article 10.1016/j.biomaterials.2011.09.064 1 BIOMATERIALS, v.33, no.3, pp.856 - 866 BIOMATERIALS 33 3 856 866 scie scopus 000298212400014 2-s2.0-81155128532 Engineering, Biomedical Materials Science, Biomaterials Engineering Materials Science Article TARGETED DELIVERY CELLS ACID PEPTIDE TAXOL Gold nanoparticles Paclitaxel Biotin Beta-cyclodextrin Theranostic agents