Choi, Lee Kwak, Soon Jong You, Su Jung Chun, Heung Jae Kim, Hong Lim Shim, Young Bock Kim, Moon Suk Park, Ki Dong 2024-01-20T15:34:18Z 2024-01-20T15:34:18Z 2021-09-05 2012-01 1598-5032 https://pubs.kist.re.kr/handle/201004/129690 The present study demonstrated that covalently galactosylated poly(D,L-lactic-co-glycolic acid) (PLGA) surface encourages hepatocyte adhesion and growth to form a dense cell network. Galactosylation of the PLGA surface was accomplished by grafting allylamine (AA) using inductively coupled plasma-assisted chemical vapor deposition (ICP-CVD) and conjugating lactobionic acid (LA) with AA via 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide and N-hydroxysuccinimide (EDC/NHS) activation. The modified surface was characterized by Fourier transform infrared spectroscopy in the attenuated total reflectance, electron spectroscopy of chemical analysis, atomic force microscopy, and contact angle measurement. For evaluation of cell affinity in vitro, primary rat hepatocytes were prepared and seeded onto the modified PLGA surfaces. The galactosylated PLGA surface showed more pronounced hepatocyte adhesion and growth compared to those on the control PLGA surface. The hepatocytes seeded on galactosylated substrates exhibited a radial migration with filopodial growth to form multicellular aggregates, whereas those on control PLGA showed slowly adhered rounded shapes. Moreover, galactosylation increased metabolic hepatocyte activities such as albumin secretion and urea synthesis. English SPRINGER MODIFIED POLY(DL-LACTIDE-CO-GLYCOLIDE) SCAFFOLDS HYDROPHILIC MONOMERS SPHEROID FORMATION CELLS IMMOBILIZATION ATTACHMENT POLYMER Characterization and hepatocytes adhesion of galactosylated poly(D,L-lactic-co-glycolic acid) surface Article 10.1007/s13233-012-0005-6 1 MACROMOLECULAR RESEARCH, v.20, no.1, pp.93 - 100 MACROMOLECULAR RESEARCH 20 1 93 100 scie scopus kci ART001610189 000298883100013 2-s2.0-84856214485 Polymer Science Polymer Science Article MODIFIED POLY(DL-LACTIDE-CO-GLYCOLIDE) SCAFFOLDS HYDROPHILIC MONOMERS SPHEROID FORMATION CELLS IMMOBILIZATION ATTACHMENT POLYMER poly(D,L-lactic-co-glycolic acid) (PLGA) allylamine inductively coupled plasma-assisted chemical vapor deposition (ICP-CVD) galactose ligand hepatocyte multicellular aggregates