So, Young-Jin Shin, Chan-Young Song, Mee Rha, Young-Ah Ryu, Jae-Chun 2024-01-20T15:34:36Z 2024-01-20T15:34:36Z 2021-09-05 2011-12-31 1738-642X https://pubs.kist.re.kr/handle/201004/129703 Para-phenylenediamine (PPD) is widely used in a variety of products, including hair dyes. Sensitization to PPD is a well-known cause of allergic contact dermatitis. However, the molecular mechanism of PPD-induced cellular toxicity remains unclear. We performed a genome-wide analysis of transcriptional responses of human HaCaT keratinocytes to an IC20 dose of PPD (60 mu M). PPD downregulated the expression of 650 genes and upregulated the expression of 854 genes in the keratinocytes. PPD-responsive genes were involved in inflammatory responses, responses to oxidative stress, Toll-like receptor signaling, and metabolism of xenobiotics by cytochrome P450. Our results indicate that changes in gene expression are associated with PPD-induced skin toxicity, and the genes identified may be promising biomarkers of PPD-induced skin toxicity. Possible roles of the responsive genes and related pathways in PPD-induced cellular responses are discussed. This study helps to elucidate cellular changes in transcriptional regulation in response to PPD exposure. English KOREAN SOCIETY TOXICOGENOMICS & TOXICOPROTEOMICS-KSTT OXIDATIVE STRESS CONTACT SENSITIZERS P-PHENYLENEDIAMINE BLADDER-CANCER N-ACETYLATION DYE USE SKIN PARAPHENYLENEDIAMINE ASSOCIATION ACTIVATION Gene expression profiling of hair-dying agent, para-phenylenediamine, in human keratinocytes (HaCaT) cells Article 10.1007/s13273-011-0043-8 1 MOLECULAR & CELLULAR TOXICOLOGY, v.7, no.4, pp.339 - 346 MOLECULAR & CELLULAR TOXICOLOGY 7 4 339 346 scie scopus kci other ART001661241 000298789700002 2-s2.0-84856301015 Biochemistry & Molecular Biology Toxicology Biochemistry & Molecular Biology Toxicology Article OXIDATIVE STRESS CONTACT SENSITIZERS P-PHENYLENEDIAMINE BLADDER-CANCER N-ACETYLATION DYE USE SKIN PARAPHENYLENEDIAMINE ASSOCIATION ACTIVATION Para-phenylenediamine(PPD 1,4-diaminobenzene CAS 106-50-3) Keratinocytes Microarray Gene ontology (GO)