Hwang, Kyo Seon Lee, Min Hyuck Lee, Juhee Yeo, Woon-Seok Lee, Jeong Noon Kim, Kang-Min Kang, Ji Yoon Kim, Tae Song 2024-01-20T15:34:49Z 2024-01-20T15:34:49Z 2021-09-05 2011-12-15 0956-5663 https://pubs.kist.re.kr/handle/201004/129714 We reported that peptide could be utilized as receptor molecule in the gas phase for application in micro/nano sensors by using a specific peptide that recognizes 2,4-dinitrotoluene at room temperature and in an atmospheric environment and measuring changes in the resonant frequency of the peptide immobilized microcantilevers. By using these peptides as receptors on a microcantilever sensor, we were able to experimentally detect 2,4-dinitrotoluene (DNT) vapor at concentrations as low as parts per billion (ppb) in the gas phase. While resonant frequency changes after binding between 2,4-DNT and the specific peptide receptor that was immobilized on microcantilevers were observed, the resonant frequency of DNT nonspecific peptide immobilized microcantilever did not change when exposed to 2,4-DNT vapor. The limit of detection (LOD) was calculated to be 431 ppt of limit of detection is numerically expected by experimental based on an equation that describes the relationship between the noise-equivalent analyte concentration. These results indicate that the peptide receptors hold great promise for use in the development of an artificial olfactory system and electronic nose based on micro/nanotechnology for monitoring various chemical vapors in the gas phase such as explosive mixtures of chemicals and/or volatile organic compounds. (C) 2011 Elsevier B.V. All rights reserved. English ELSEVIER ADVANCED TECHNOLOGY RESONANT-FREQUENCY SHIFT LABEL-FREE DETECTION CANTILEVER ARRAY MASS PROTEINS SYSTEMS Peptide receptor-based selective dinitrotoluene detection using a microcantilever sensor Article 10.1016/j.bios.2011.09.021 1 BIOSENSORS & BIOELECTRONICS, v.30, no.1, pp.249 - 254 BIOSENSORS & BIOELECTRONICS 30 1 249 254 scie scopus 000297610200038 2-s2.0-80755186520 Biophysics Biotechnology & Applied Microbiology Chemistry, Analytical Electrochemistry Nanoscience & Nanotechnology Biophysics Biotechnology & Applied Microbiology Chemistry Electrochemistry Science & Technology - Other Topics Article RESONANT-FREQUENCY SHIFT LABEL-FREE DETECTION CANTILEVER ARRAY MASS PROTEINS SYSTEMS Microcantilever Resonant frequency Dinitrotoluene Electronic nose Artificial olfactory system