Moon, Ju-Yeon Kang, Se Mi Moon, Myeong Hee Hong, Jongki Kim, Ki Tae Jeong, Dae Hoon Kim, Young Nam Chung, Bong Chul Choi, Man Ho 2024-01-20T15:35:01Z 2024-01-20T15:35:01Z 2021-09-05 2011-12-01 1570-0232 https://pubs.kist.re.kr/handle/201004/129723 A comprehensive gas chromatography-mass spectrometry (GC-MS)-based profiling was developed as a practical assay for quantification of 18 endogenous estrogens in serum samples. The present GC-MS method was conducted with the two-phase extractive ethoxycarbonlyation (EOC) of the phenolic hydroxy groups of estrogen with ethyl chlorformate combined with the non-polar n-hexane extraction. The subsequent perfluoroacylation of aliphatic hydroxy groups with pentafluoropropionyl anhydride (PFPA) was conducted. The serum samples were separated through a high temperature GC column (MXT-1) within an 8-min run and analyzed in selected-ion monitoring mode with good chromatographic properties for 18 estrogens as their EOC-PFP derivatives. The limit of quantification (LOQ) was 0.025-0.10 ng/mL for most estrogens analyzed except for E3 and 2-OH-E3 (0.5 ng/mL each). The devised method was found to be linear over a 10(3)-fold concentration range with a correlation coefficient (r(2) > 0.992), whereas the precision (% CV) and accuracy (% bias) ranged from 3.1 to 16.3% and from 93.5 to 111.1%, respectively. Decreased 2-methoxy-17 beta-estradiol levels were confirmed in patients with preeclampsia than healthy pregnant women. This technique can be used for a clinical diagnosis as well as understanding the pathogenesis in estrogen-related disorders. (C) 2011 Elsevier B.V. All rights reserved. English ELSEVIER CATECHOL-O-METHYLTRANSFERASE ESTRADIOL ESTRONE METABOLISM CANCER 17-BETA-ESTRADIOL DEFICIENCY CELLS HAIR RIA Extractive ethoxycarbonylation in high-temperature gas chromatography mass spectrometry based analysis of serum estrogens Article 10.1016/j.jchromb.2011.10.024 1 JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, v.879, no.31, pp.3742 - 3748 JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 879 31 3742 3748 scie scopus 000297830600011 2-s2.0-82955182194 Biochemical Research Methods Chemistry, Analytical Biochemistry & Molecular Biology Chemistry Article CATECHOL-O-METHYLTRANSFERASE ESTRADIOL ESTRONE METABOLISM CANCER 17-BETA-ESTRADIOL DEFICIENCY CELLS HAIR RIA Estrogen Serum GC-MS Extractive ethoxycarbonylation Preeclampsia