The Han Nguyen Um, Byung Hun Kim, Sang Moo 2024-01-20T16:02:17Z 2024-01-20T16:02:17Z 2021-09-05 2011-11 0022-1147 https://pubs.kist.re.kr/handle/201004/129829 One therapeutic approach for preventing diabetes mellitus and obesity is to retard the absorption of glucose via inhibition of a-glucosidase. Two unsaturated fatty acids with strong a-glucosidase inhibitory activity, 7(Z)-octadecenoic acid (1) and 7(Z),10(Z)-octadecadienoic acid (2), were purified from the body wall of Stichopus japonicus. IC50 values of compounds 1 and 2 were 0.51 and 0.67 mu g/mL against Saccharomyces cerevisiae a-glucosidase and 0.49 and 0.60 mu g/mL against Bacillus stearothermophilus a-glucosidase, respectively. These compounds mildly inhibited rat-intestinal sucrase and maltase. In addition, both compounds showed a mixed type of inhibition against S. cerevisiae a-glucosidase and were very stable under thermal and acidic conditions up to 60 min. The KI and KIS values of compounds 1 and 2 were 0.44 and 0.22 mu g/mL, and 0.39 and 0.13 mu g/mL, respectively. English WILEY BOVINE SERUM-ALBUMIN MUSHROOM TYROSINASE ENZYME MECHANISM SAPONIN BINDING Two Unsaturated Fatty Acids with Potent alpha-Glucosidase Inhibitory Activity Purified from the Body Wall of Sea Cucumber (Stichopus japonicus) Article 10.1111/j.1750-3841.2011.02391.x 1 JOURNAL OF FOOD SCIENCE, v.76, no.9, pp.H208 - H214 JOURNAL OF FOOD SCIENCE 76 9 H208 H214 scie scopus 000297457000010 2-s2.0-80955148936 Food Science & Technology Food Science & Technology Article BOVINE SERUM-ALBUMIN MUSHROOM TYROSINASE ENZYME MECHANISM SAPONIN BINDING diabetes fatty acid a-glucosidase inhibitor kinetics sea cucumber