Choi, Kyung-mi Choi, Seung-Hye Jeon, Hyesung Kim, In-San Ahn, Hyung Jun 2024-01-20T16:02:36Z 2024-01-20T16:02:36Z 2021-09-05 2011-11 1936-0851 https://pubs.kist.re.kr/handle/201004/129846 For the efficient cytoplasmic delivery of siRNA, we designed a chimeric capsid protein composed of a capsid shell, integrin targeting peptide, and p19 RNA binding protein. This recombinant protein assembled into a macromolecular container-like structure with capsid shell and provided a nanocarrier for siRNA delivery. Our capsid nanocarriers had dual affinity both for siRNA within the interior and Integin receptors on the exterior, and the capsid shell structure allowed the encapsulated siRNAs to be protected from the external nucleases, leading to the enhanced stability of siRNA in serum conditions. The capsid nanocarriers could complex with siRNA in a size-dependent and sequence-independent manner and showed the pH-dependent complexing/dissocation behaviors with siRNA. Moreover, RGD peptides on the exterior surface of the capsid shell enabled the capsid nanocarriers to deliver siRNA into the cytosol of the target cells. Here, we demonstrated the superior efficiency of our siRNA/capsid nanocarrier complexes in RFP gene silencing, compared to untreated cells. These results provide an alternative approach to enhancing the stability of siRNA as well as to achieving targeted siRNA delivery. English AMER CHEMICAL SOC SMALL INTERFERING RNA GENE DELIVERY IN-VIVO ELECTRON CRYOMICROSCOPY SYNTHETIC SIRNAS MAMMALIAN-CELLS COATED PIT VECTORS EFFICIENT CYTOTOXICITY Chimeric Capsid Protein as a Nanocarrier for siRNA Delivery: Stability and Cellular Uptake of Encapsulated siRNA Article 10.1021/nn202597c 1 ACS NANO, v.5, no.11, pp.8690 - 8699 ACS NANO 5 11 8690 8699 scie scopus 000297143300027 2-s2.0-81855177550 Chemistry, Multidisciplinary Chemistry, Physical Nanoscience & Nanotechnology Materials Science, Multidisciplinary Chemistry Science & Technology - Other Topics Materials Science Article SMALL INTERFERING RNA GENE DELIVERY IN-VIVO ELECTRON CRYOMICROSCOPY SYNTHETIC SIRNAS MAMMALIAN-CELLS COATED PIT VECTORS EFFICIENT CYTOTOXICITY capsid protein siRNA delivery nanocarrier gene silencing recombinant proteins