Moon, Chuloo Lee, Chang Hwan Sang, Byoung-In Um, Youngsoon 2024-01-20T16:02:40Z 2024-01-20T16:02:40Z 2021-09-05 2011-11 0960-8524 https://pubs.kist.re.kr/handle/201004/129849 Medium compositions favoring butanol and 1,3-propanediol (1,3-PDO) production from glycerol by Clostridium pasteurianurn DSM525 were investigated using statistical experimental designs. Medium components affecting butanol and 1,3-PDO production were screened using a fractional factorial experimental design. Among the six tested variables (phosphate buffer, MnSO4 center dot H2O, MgSO4 center dot 7H(2)O, FeSO4 center dot 7H(2)O, (NH4)(2)SO4, and yeast extract), FeSO4 center dot 7H(2)O, (NH4)(2)SO4, and yeast extract were found to be significant variables for further optimization of medium using a Box-Behnken design. Optimal butanol (0.98 g/L/h) and 1,3-PDO (1.19 g/L/h) productivities were predicted by the corresponding quadratic model for each product and the models were validated experimentally under optimized conditions. The optimal medium composition for butanol production was significantly different from that for 1,3-PDO production (0.06 vs. 0 g/L for FeSO4 center dot 7H(2)O, 7.35 vs. 0 g/L for (NH4)(2)SO4, and 5.08 vs. 8.0 g/L for yeast extract), suggesting that the product formation from glycerol by C. pasteurianum DSM525 can be controlled by changing medium compositions. (C) 2011 Elsevier Ltd. All rights reserved. English ELSEVIER SCI LTD Optimization of medium compositions favoring butanol and 1,3-propanediol production from glycerol by Clostridium pasteurianum Article 10.1016/j.biortech.2011.08.094 1 BIORESOURCE TECHNOLOGY, v.102, no.22, pp.10561 - 10568 BIORESOURCE TECHNOLOGY 102 22 10561 10568 N scie scopus 000297657400059 2-s2.0-80054835190 Agricultural Engineering Biotechnology & Applied Microbiology Energy & Fuels Agriculture Biotechnology & Applied Microbiology Energy & Fuels Article SOLVENT PRODUCTION FERMENTATION ETHANOL Response surface methodology Glycerol Butanol 1,3-Propanediol Clostridium pasteurianum