Ryu, Ju Hee Lee, Aeju Na, Jin Hee Lee, Seulki Ahn, Hyung Jun Park, Jong Woong Ahn, Cheol-Hee Kim, Byung-Soo Kwon, Ick Chan Choi, Kuiwon Youn, Inchan Kim, Kwangmeyung 2024-01-20T16:02:43Z 2024-01-20T16:02:43Z 2021-09-05 2011-11 0939-4451 https://pubs.kist.re.kr/handle/201004/129852 Among the classical collagenases, matrix metalloproteinase-13 (called MMP-13, collagenase-3) is one of the most important components for cartilage destruction of osteoarthritis (OA) developments. Despite many efforts, the detection methods of MMP-13 activity have been met with limited success in vivo, in part, due to the low sensitivity and low selectivity by homology of MMP family. Previously, we demonstrated the use of strongly dark-quenched fluorogenic probe allowed for the visual detection of MMP-13 in vitro and in OA-induced rat models. In this study, we described the optimization of MMP-13 fluorogenic probe for OA detection in vivo. Three candidate probes demonstrated recovered fluorescent intensity proportional with MMP-13 concentrations, respectively; however, Probe 2 exhibited both high signal amplification and selective recognition for MMP-13, not MMP-2 and MMP-9 in vitro. When Probe 2 was applied to OA-induced rat models, clear visualization of MMP-13 activity in OA-induced cartilage was obtained. Optimized MMP-13 fluorogenic probe can be applied to detect and image OA and have potential for evaluating the in vivo efficacy of MMP-13 inhibitors which are being tested for therapeutic treatment of OA. English SPRINGER RHEUMATOID-ARTHRITIS CARTILAGE DEGRADATION EXTRACELLULAR-MATRIX IN-VIVO PEPTIDE EXPRESSION COLLAGENASE ACTIVATION INHIBITOR PROTEINS Optimization of matrix metalloproteinase fluorogenic probes for osteoarthritis imaging Article 10.1007/s00726-010-0769-y 1 AMINO ACIDS, v.41, no.5, pp.1113 - 1122 AMINO ACIDS 41 5 1113 1122 scie scopus 000296469100010 2-s2.0-84855223512 Biochemistry & Molecular Biology Biochemistry & Molecular Biology Article RHEUMATOID-ARTHRITIS CARTILAGE DEGRADATION EXTRACELLULAR-MATRIX IN-VIVO PEPTIDE EXPRESSION COLLAGENASE ACTIVATION INHIBITOR PROTEINS Matrix metalloproteinase (MMP) Osteoarthritis Fluorogenic probe Optical imaging Peptide