Zhu, Lei Zhang, Fan Ma, Ying Liu, Gang Kim, Kwangmeyung Fang, Xuexun Lee, Seulki Chen, Xiaoyuan 2024-01-20T16:02:45Z 2024-01-20T16:02:45Z 2021-09-04 2011-11 1543-8384 https://pubs.kist.re.kr/handle/201004/129854 Herein we demonstrate for the first time that a fluorogenic probe can be used as an in vivo imaging agent for visualizing activities of membrane-tethered, membrane-type matrix metalloproteinases (MT-MMPs). An MT-MMP fluorogenic probe that consisted of an MT1-MMP (MMP-14) substrate and near-infrared (NIR) dye-quencher pair exhibited rapid, efficient boosts in fluorescence upon cleavage by MT1-MMP in tumor-bearing mice. In particular, unlike similar fluorogenic probes designed to target extracellular, soluble-type MMPs (EC-MM.Ps) which can be cleared from the bloodstream after activation-the fluorescence signals activated by MT1-MIMP enable clear visualization of MT1-MMP-positive tumors in animal models for up to 24 h. The results indicate that a simple form of a fluorogenic probe that is less effective in EC-MMP imaging is an effective probe for imaging MT-MMP activities in vivo. These findings can be widely applied to designing probes and to applications targeting various membrane-anchored proteases in vivo. English AMER CHEMICAL SOC CELL PENETRATING PEPTIDES THERAPEUTIC TARGET FLUOROGENIC PROBE CANCER INHIBITORS MT1-MMP BEACON In Vivo Optical Imaging of Membrane-Type Matrix Metalloproteinase (MT-MMP) Activity Article 10.1021/mp2002297 1 MOLECULAR PHARMACEUTICS, v.8, no.6, pp.2331 - 2338 MOLECULAR PHARMACEUTICS 8 6 2331 2338 scie scopus 000297537300035 2-s2.0-82955169662 Medicine, Research & Experimental Pharmacology & Pharmacy Research & Experimental Medicine Pharmacology & Pharmacy Article CELL PENETRATING PEPTIDES THERAPEUTIC TARGET FLUOROGENIC PROBE CANCER INHIBITORS MT1-MMP BEACON activatable probe fluorogenic probe membrane-type matrix metalloproteinase optical imaging protease