Hong, Yu Jin Bae, Soon Eon Do, Sun Hee Kim, Ik Hwan Han, Dong Keun Park, Kwideok 2024-01-20T16:04:17Z 2024-01-20T16:04:17Z 2021-09-05 2011-10 1598-5032 https://pubs.kist.re.kr/handle/201004/129923 A cell-derived extracellular matrix (ECM) was naturally obtained and its effect on the induction of osteogenesis of bone marrow stromal cells (BMSCs) was investigated. Once porous composite scaffolds made of poly(L-lactic-co-glycolic acid) (PLGA), hydroxyapatite (HA), and beta-tricalcium phosphate (beta-TCP) were fabricated, these scaffolds were seeded with fibroblasts or preosteoblasts and cultured in vitro. They were then subjected to decellularization, resulting in fibroblasts-decellularized scaffolds (FDS) or preosteoblasts-decellularized scaffolds (PDS). Both fibronectin and type I collagen were clearly detected from the immunofluorescent staining of FDS and PDS, respectively. When the rabbit BMSCs-loaded scaffolds were cultured in the osteogenic medium for 4 weeks, the osteogenic potential of FDS and PDS was much greater than the PLGA/HA/beta-TCP (control), as identified by histological staining and the alkaline phosphatase (ALP) activity. Meanwhile, when BMSC-seeded FDS was implanted subcutaneously into the nude mice, the results also indicated more upregulated osteogenic differentiation of BMSCs in vivo compared to the control. This study suggests that the microenvironment created by cell-derived ECM can provide a favorable template in prompting the osteogenesis of BMSCs. English POLYMER SOC KOREA MESENCHYMAL STEM-CELLS EXTRACELLULAR-MATRIX DIFFERENTIATION GROWTH HEART Decellularized PLGA-based scaffolds and their osteogenic potential with bone marrow stromal cells Article 10.1007/s13233-011-1004-8 1 MACROMOLECULAR RESEARCH, v.19, no.10, pp.1090 - 1096 MACROMOLECULAR RESEARCH 19 10 1090 1096 scie scopus kci ART001584611 000294812500015 2-s2.0-80052651596 Polymer Science Polymer Science Article MESENCHYMAL STEM-CELLS EXTRACELLULAR-MATRIX DIFFERENTIATION GROWTH HEART bone marrow stromal cells osteogenic differentiation extracellular matrix scaffold decellularization