Chi Hoa Pham Park, Kyung Seo Kim, Byoung Chan Kim, Han Na Gu, Man Bock 2024-01-20T16:04:47Z 2024-01-20T16:04:47Z 2021-09-05 2011-10 0166-445X https://pubs.kist.re.kr/handle/201004/129949 To strengthen the toxicogenomic study, we constructed a library of hepatic cDNA from Japanese medaka under influence of specific chemical mediated stress responses. Gene expression profile analysis of the cDNA microarrays followed by real time RT-PCR assay were conducted to screen particular biomarkers for 17-beta estradiol (E2), nonylphenol (NP) and 2-chlorophenol (2CP). Information of 1509 high-quality ESTs including 260 new ESTs was added onto GenBank and dbEST. The ESTs were clustered and assembled into 159 contigs and 372 singletons. Among them, 128 contigs and 163 singletons (54.8%) were functionally characterized and 13 UniESTs (2.5%) were hypothetical proteins. Ontology analysis resulting in 282 UniESTs which involved with 2102 GOs and 93 sequences associated with 116 enzyme codes. For each test chemical, two specific biomarkers were selected from the gene expression profiling of microarrays. The expression patterns of the marker genes in real time PCR analysis were consistent with the regulated gene expression patterns in microarrays. The tentative biomarkers showed unique gene expression patterns depending on chemical concentration(s) and exposure duration in real time RT-PCR analysis. The analysis accomplished of the hepatic cDNA library and its information added to genetic and genomic resources could be sufficiently valuable specifically for aquatic toxicity studies. (C) 2011 Elsevier B.V. All rights reserved. English ELSEVIER GENE-EXPRESSION RESOURCE BETA TOOL EST ANNOTATION EXPOSURE GENOMICS ESTROGEN ALPHA Construction and characterization of Japanese medaka (Oryzias latipes) hepatic cDNA library and its implementation to biomarker screening in aquatic toxicology Article 10.1016/j.aquatox.2011.08.019 1 AQUATIC TOXICOLOGY, v.105, no.3-4, pp.569 - 575 AQUATIC TOXICOLOGY 105 3-4 569 575 scie scopus 000298120600041 2-s2.0-80052851016 Marine & Freshwater Biology Toxicology Marine & Freshwater Biology Toxicology Article GENE-EXPRESSION RESOURCE BETA TOOL EST ANNOTATION EXPOSURE GENOMICS ESTROGEN ALPHA cDNA library Aquatic toxicology Medaka Biomarker Real time RT-PCR