Zhao, Shengli Ting, Jonathan T. Atallah, Hisham E. Qiu, Li Tan, Jie Gloss, Bernd Augustine, George J. Deisseroth, Karl Luo, Minmin Graybiel, Ann M. Feng, Guoping 2024-01-20T16:30:46Z 2024-01-20T16:30:46Z 2022-01-10 2011-09 1548-7091 https://pubs.kist.re.kr/handle/201004/130005 Optogenetic methods have emerged as powerful tools for dissecting neural circuit connectivity, function and dysfunction. We used a bacterial artificial chromosome (BAC) transgenic strategy to express the H134R variant of channelrhodopsin-2, ChR2(H134R), under the control of cell type-specific promoter elements. We performed an extensive functional characterization of the newly established VGAT-ChR2(H134R)-EYFP, ChAT-ChR2(H134R)-EYFP, Tph2-ChR2(H134R)-EYFP and Pvalb(H134R)-ChR2-EYFP BAC transgenic mouse lines and demonstrate the utility of these lines for precisely controlling action-potential firing of GABAergic, cholinergic, serotonergic and parvalbumin-expressing neuron subsets using blue light. This resource of cell type-specific ChR2(H134R) mouse lines will facilitate the precise mapping of neuronal connectivity and the dissection of the neural basis of behavior. English NATURE PUBLISHING GROUP THALAMIC RETICULAR NUCLEUS RAT-BRAIN SLICES CHOLINERGIC INTERNEURONS CORELEASE GLUTAMATE SPINAL-CORD IN-VIVO NEURONS ACTIVATION BEHAVIORS RESPONSES Cell type-specific channelrhodopsin-2 transgenic mice for optogenetic dissection of neural circuitry function Article 10.1038/nmeth.1668 1 NATURE METHODS, v.8, no.9, pp.745 - U91 NATURE METHODS 8 9 745 U91 scie scopus 000294439100011 2-s2.0-80052282876 Biochemical Research Methods Biochemistry & Molecular Biology Article THALAMIC RETICULAR NUCLEUS RAT-BRAIN SLICES CHOLINERGIC INTERNEURONS CORELEASE GLUTAMATE SPINAL-CORD IN-VIVO NEURONS ACTIVATION BEHAVIORS RESPONSES