Kim, Sunoh Rhim, Hyewhon 2024-01-20T16:30:54Z 2024-01-20T16:30:54Z 2021-09-05 2011-09 1016-8478 https://pubs.kist.re.kr/handle/201004/130012 Overload of intracellular Ca2+ has been implicated in the pathogenesis of neuronal disorders, such as Alzheimer's disease. Various mechanisms produce abnormalities in intracellular Ca2+ homeostasis systems. L-type Ca2+ channels have been known to be closely involved in the mechanisms underlying the neurodegenerative properties of amyloid-beta (A beta) peptides. However, most studies of L-type Ca2+ channels in A beta-related mechanisms have been limited to Ca(V)1.2, and surprisingly little is known about the involvement of Ca(V)1.3 in A beta-induced neuronal toxicity. In the present study, we examined the expression patterns of Ca(V)1.3 after A beta(25-35) exposure for 24 h and compared them with the expression patterns of Ca(V)1.2. The expression levels of Ca(V)1.3 were not significantly changed by A beta(25-35) at both the mRNA levels and the total protein level in cultured hippocampal neurons. However, surface protein levels of Ca(V)1.3 were significantly increased by A beta(25-35), but not by A beta(35-25). We next found that acute treatment with A beta(25-35) increased Ca(V)1.3 channel activities in HEK293 cells using whole-cell patch-clamp recordings. Furthermore, using GTP pulldown and co-immunoprecipitation assays in HEK293 cell lysates, we found that amyloid precursor protein interacts with beta(3) subunits of Ca2+ channels instead of Ca(V)1.2 or Ca(V)1.3 alpha(1) subunits. These results show that A beta(25-35) chronically or acutely upregulates Ca(V)1.3 in the rat hippocampal and human kidney cells (HEK293). This suggests that Ca(V)1.3 has a potential role along with Ca(V)1.2 in the pathogenesis of Alzheimer's disease. English KOREAN SOC MOLECULAR & CELLULAR BIOLOGY CULTURED HIPPOCAMPAL-NEURONS CALCIUM-CHANNEL ALZHEIMERS-DISEASE CELL-DEATH EXPRESSION RECEPTOR DYSREGULATION BLOCKADE INFLUX Effects of Amyloid-beta Peptides on Voltage-Gated L-Type Ca(V)1.2 and Ca(V)1.3 Ca2+ Channels Article 10.1007/s10059-011-0075-x 1 MOLECULES AND CELLS, v.32, no.3, pp.289 - 294 MOLECULES AND CELLS 32 3 289 294 scie scopus kci ART001591901 000297627300011 2-s2.0-84862908515 Biochemistry & Molecular Biology Cell Biology Biochemistry & Molecular Biology Cell Biology Article CULTURED HIPPOCAMPAL-NEURONS CALCIUM-CHANNEL ALZHEIMERS-DISEASE CELL-DEATH EXPRESSION RECEPTOR DYSREGULATION BLOCKADE INFLUX Alzheimer&apos s disease co-immunoprecipitation GST pulldown intracellular Ca2+ L-type Ca2+ channels