Jung, So-Ra Song, No-Joon Hwang, Hyun Sook An, Jae Jin Cho, Yong-Jun Kweon, Hae Young Kang, Seok-Woo Lee, Kwang Gill Yoon, Keejung Kim, Byung-Joon Nho, Chu Won Choi, Soo Young Park, Kye Won 2024-01-20T16:31:09Z 2024-01-20T16:31:09Z 2021-09-05 2011-09 0271-5317 https://pubs.kist.re.kr/handle/201004/130024 Silk protein is a biocompatible material that has been used in many biotechnological applications and exhibits body fat lowering effects. Recent studies have shown that silk peptides increase expression of osteogenic markers in osteoblast-like cells. Because osteogenic and adipogenic differentiation from common mesenchymal progenitor cells are inverse processes and often regulated reciprocally, we hypothesized that silk peptides might suppress adipocyte differentiation. We therefore endeavored to evaluate the effects of silk peptides on adipocyte differentiation in C3H10T1/2 cells. We find that silk peptides inhibit lipid accumulation and morphological differentiation in these cells. Molecular studies show that silk peptides block expression of adipocyte-specific genes such as peroxisome proliferator-activated receptor gamma and its targets, including aP2, Cd36, CCAAT enhancer binding protein alpha. Silk peptides appear to inhibit adipogenesis by suppression of the Notch pathway, repressing the Notch target genes Hes-1 and Hey-1. In addition, these peptides inhibit endogenous Notch activation, as shown by a reduction in generation of Notch intracellular domain. N-[N-(3.5-difluorophenacetyl)-L-alanyl]-S-phenylglycine t-butylester, compound E, and WPE-III-31C, which are all known Notch signaling inhibitors, block adipocyte differentiation to an extent similar to silk peptides. Together, our data demonstrate that silk peptides can modulate adipocyte differentiation through inhibition of the Notch signaling and further suggest potential future strategies for treating obesity and its related metabolic diseases. (C) 2011 Elsevier Inc. All rights reserved. English PERGAMON-ELSEVIER SCIENCE LTD MESENCHYMAL STEM-CELLS HUMAN BONE-MARROW FIBROIN 3D SCAFFOLDS OSTEOBLAST DIFFERENTIATION PPAR-GAMMA IN-VITRO ADIPOGENESIS TISSUE FAT PROLIFERATION Silk peptides inhibit adipocyte differentiation through modulation of the Notch pathway in C3H10T1/2 cells Article 10.1016/j.nutres.2011.08.010 1 NUTRITION RESEARCH, v.31, no.9, pp.723 - 730 NUTRITION RESEARCH 31 9 723 730 scie scopus 000296671900009 2-s2.0-82555204698 Nutrition & Dietetics Nutrition & Dietetics Article MESENCHYMAL STEM-CELLS HUMAN BONE-MARROW FIBROIN 3D SCAFFOLDS OSTEOBLAST DIFFERENTIATION PPAR-GAMMA IN-VITRO ADIPOGENESIS TISSUE FAT PROLIFERATION Adipocyte differentiation PPAR gamma C3H10T1/2 cells Silk Notch pathway