Jeon, Byoung Wook Yoo, Hye Hyun Jeong, Eun Sook Kim, Ho Jun Jin, Changbae Kim, Dong Hyun Lee, Jaeick 2024-01-20T16:31:31Z 2024-01-20T16:31:31Z 2021-09-05 2011-09 1618-2642 https://pubs.kist.re.kr/handle/201004/130043 A sensitive and rapid method based on liquid chromatography-triple-quadrupole tandem mass spectrometry (LC-MS/MS) with electrospray ionization (ESI) has been developed and validated for the screening and confirmation of 44 exogenous anabolic steroids (29 parent steroids and 15 metabolites) in human urine. The method involves an enzymatic hydrolysis, liquid-liquid extraction, and detection by LC-MS/MS. A triple-quadrupole mass spectrometer was operated in positive ESI mode with selected reaction monitoring (SRM) mode for the screening and product ion scan mode for the confirmation. The protonated molecular ions were used as precursor ions for the SRM analysis and product ion scan. The intraday and interday precisions of the target analytes at concentrations of the minimum required performance levels for the screening were 2-14% and 2-15%, respectively. The limits of detection for the screening and confirmation method were 0.1-10 ng/mL and 0.2-10 ng/mL, respectively, for 44 steroids. This method was successfully applied to analysis of urine samples from suspected anabolic steroid abusers. English SPRINGER HEIDELBERG TANDEM MASS-SPECTROMETRY LIQUID-CHROMATOGRAPHY EQUINE PLASMA QUANTIFICATION AGENTS DRUGS LC-ESI/MS/MS method for rapid screening and confirmation of 44 exogenous anabolic steroids in human urine Article 10.1007/s00216-011-5210-1 1 ANALYTICAL AND BIOANALYTICAL CHEMISTRY, v.401, no.4, pp.1353 - 1363 ANALYTICAL AND BIOANALYTICAL CHEMISTRY 401 4 1353 1363 scie scopus 000293632700029 2-s2.0-80052603242 Biochemical Research Methods Chemistry, Analytical Biochemistry & Molecular Biology Chemistry Article TANDEM MASS-SPECTROMETRY LIQUID-CHROMATOGRAPHY EQUINE PLASMA QUANTIFICATION AGENTS DRUGS Anabolic steroids Screening Confirmation Doping analysis Liquid chromatography-tandem mass spectrometry