Sarma, Sailendra Nath Kim, Youn-Jung Ryu, Jae-Chun 2024-01-20T16:31:43Z 2024-01-20T16:31:43Z 2021-09-04 2011-09 1382-6689 https://pubs.kist.re.kr/handle/201004/130053 Benzene is a well-known environmental pollutant that can induce hematotoxicity, aplastic anemia, acute myelogenous leukemia, and lymphoma. Benzene toxicity is likely mediated through metabolites induced by means of multiple pathways. Although benzene metabolites are known to induce oxidative stress and disrupt the cell cycle, the mechanism underlying leukemogenesis is not fully understood. The aim of this study was to analyze the genome-wide expression profiles of human promyelocytic leukemia HL-60 cells that had been exposed to benzene and its metabolites. This was carried out using whole human genome oligonucleotide microarrays to ascertain potential biomarkers. Genes that were differentially expressed (>1.5-fold and p-values <0.05) after exposure to benzene (BZ), hydroquinone (HQ), and 1,4-benzoquinone (BQ) were then classified with GO, KEGG and GSEA pathway annotation. All genes that were identified were then functionally categorized as being involved in the cell cycle, the p53 signaling pathway, apoptosis, the MAPK signaling pathway, or the T cell receptor signaling pathway. Functionally important genes were further validated by means of real-time RT-PCR. The results showed that EGR1, PMAIP1, AR, CCL2, CD69, HSPA8, SLC7A11, HERPUD1, ELK1, and MKI57 genes altered their expression profiles. Similar expression profiles were also found in human erythromyeloblastoid leukemia K562 cells and in human leukemic monocyte lymphoma 11937 cells. In conclusion, gene expression profiles along with GO, KEGG and GSEA pathway annotation analysis have provided an insight into the leukemogenesis as well as highlighted potential gene-based biomarkers of human leukemia cell lines when they are exposed to benzene and its metabolites. (C) 2011 Elsevier B.V. All rights reserved. English ELSEVIER SCIENCE BV ENDOPLASMIC-RETICULUM STRESS TOPOISOMERASE-II IN-VIVO APOPTOSIS PROTEIN HYDROQUINONE INDUCTION TOXICITY PATHWAY GROWTH Differential gene expression profiles of human leukemia cell lines exposed to benzene and its metabolites Article 10.1016/j.etap.2011.06.001 1 ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY, v.32, no.2, pp.285 - 295 ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY 32 2 285 295 scie scopus 000294936700022 2-s2.0-80051672855 Environmental Sciences Pharmacology & Pharmacy Toxicology Environmental Sciences & Ecology Pharmacology & Pharmacy Toxicology Article ENDOPLASMIC-RETICULUM STRESS TOPOISOMERASE-II IN-VIVO APOPTOSIS PROTEIN HYDROQUINONE INDUCTION TOXICITY PATHWAY GROWTH Gene expression profiles Benzene Hydroquinone Benzoquinone Human leukemia cell