Lee, Yeon Sil Kim, Seon Ha Kim, Jin Kyu Lee, Sanghyun Jung, Sang Hoon Lim, Soon Sung 2024-01-20T16:31:54Z 2024-01-20T16:31:54Z 2021-09-01 2011-09 0958-0344 https://pubs.kist.re.kr/handle/201004/130062 Introduction - Isoflavonoids from Belamcanda chinensis are known to have a number of physiological benefits including anti-inflammatory, anti-angiogenic and anti-mutagenic properties. However, there have been no reports on the effective isolation and purification of isoflavonoids from B. chinensis. Objective - To develop an efficient method for the preparative isolation and purification of isoflavones from B. chinensis by high-speed counter-current chromatography (HSCCC). Methodology - A two-step HSCCC isolation method was developed using solvent system of n-hexane-ethyl acetate-2-propanol-methanol-water (5:6:2:3.5:6, v/v) and of ethyl acetate-methanol-water (10:2:9, v/v). FLASH purification system (45% methanol, isocratic) was also used for further purification. The purities and chemical structures of the isolated compounds were determined by high-performance liquid chromatography-photodiode array detection (HPLC-PDA), electrospray ionisation-mass spectrometry (ESI-MS), H-1- and C-13-nuclear magnetic resonance spectrometry (NMR) and nuclear overhauser enhancement (NOE). Results - HSCCC was successfully used for the preparative separation and purification of seven isoflavones, including tectoridin (145.4 mg, 97.5%), iridin (77.9 mg, 94.0%), irilin D (42.0 mg, 92.0%), tectorigenin (294.1 mg, 98.6%), iristectorigenin A (86.8 mg, 93.4%), irigenin (141.8 mg, 95.8%) and irisflorentin (73.4 mg, 94.7%) from the rhizomes of B. chinensis. Conclusion - Two isoflavone glycosides and five isoflavone derivatives were successfully isolated and purified from the crude methanol extract of dried rhizomes of the B. chinensis by HSCCC. Copyright (C) 2011 John Wiley & Sons, Ltd. English WILEY-BLACKWELL COUNTER-CURRENT CHROMATOGRAPHY ANTIOXIDANT ACTIVITIES RAPID IDENTIFICATION IRIS-TECTORUM FLAVONOIDS RHIZOMES Preparative Isolation and Purification of Seven Isoflavones from Belamcanda chinensis Article 10.1002/pca.1306 1 PHYTOCHEMICAL ANALYSIS, v.22, no.5, pp.468 - 473 PHYTOCHEMICAL ANALYSIS 22 5 468 473 scie scopus 000294443500012 2-s2.0-80054726625 Biochemical Research Methods Plant Sciences Chemistry, Analytical Biochemistry & Molecular Biology Plant Sciences Chemistry Article COUNTER-CURRENT CHROMATOGRAPHY ANTIOXIDANT ACTIVITIES RAPID IDENTIFICATION IRIS-TECTORUM FLAVONOIDS RHIZOMES Belamcanda chinensis high-speed counter-current chromatography Isoflavone