Cha, Kwang Hyun Lee, Joo Young Song, Dae-Geun Kim, Sang Min Lee, Dong-Un Jeon, Jin-Young Pan, Cheol-Ho 2024-01-20T16:32:01Z 2024-01-20T16:32:01Z 2021-09-05 2011-08-24 0021-8561 https://pubs.kist.re.kr/handle/201004/130066 Chlorella is a nutrient-rich microalga that contains protein, lipid, minerals, vitamins, and high levels of lutein. This study evaluated the bioavailability of lutein from Chlorella vulgaris using a coupled in vitro digestion and human intestinal Caco-2 cell model. Lutein bioaccessibility was low, and approximately 75% of total C. vulgaris lutein was not micellized during the digestion process but remained in the insoluble digestate. Microfluidization improved lutein micellization efficiency during C. vulgaris digestion. C. vulgaris was microfluidized at a pressure exceeding 10000 psi, and the cell surface disruption was visualized by scanning electron microscopy. The mean C. vulgaris particle size was reduced from 3.56 to 0.35 mu m with the microfluidization treatment. C. vulgaris microfluidization at 20000 psi was three times more efficient for aqueous lutein micelles production as compared with untreated C. vulgaris, and the final lutein content accumulated by intestinal Caco-2 cells was also higher with microfluidization. C. vulgaris lutein stability was not affected by microfluidization. These results indicate that microfluidization may be useful for improving lutein bioaccessibility from C. vulgaris during food processing. English AMER CHEMICAL SOC BETA-CAROTENE HETEROTROPHIC CHLORELLA DIGESTION BIOAVAILABILITY MICROALGAE BIOACCESIBILITY PROTOTHECOIDES ABSORPTION CASSAVA MEALS Effect of Microfluidization on in Vitro Micellization and Intestinal Cell Uptake of Lutein from Chlorella vulgaris Article 10.1021/jf2019243 1 JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, v.59, no.16, pp.8670 - 8674 JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 59 16 8670 8674 scie scopus 000294076600017 2-s2.0-80051738321 Agriculture, Multidisciplinary Chemistry, Applied Food Science & Technology Agriculture Chemistry Food Science & Technology Article BETA-CAROTENE HETEROTROPHIC CHLORELLA DIGESTION BIOAVAILABILITY MICROALGAE BIOACCESIBILITY PROTOTHECOIDES ABSORPTION CASSAVA MEALS Chlorella vulgaris lutein microfluidization bioaccessibility in vitro digestion micellization Caco-2 cells