Park, Hye-Won Park, Edwin H. Yun, Hyung-Mun Rhim, Hyewhon 2024-01-20T16:33:23Z 2024-01-20T16:33:23Z 2021-09-05 2011-08 0145-8884 https://pubs.kist.re.kr/handle/201004/130136 Sodium benzoate (SB) is widely used in food chemistry, cosmetics and the pharmaceutical industry. Previous studies suggest possible toxic effects of SB when used as a food preservative. However, these studies were confined to microbiological systems. Therefore, we investigated the cytotoxic effects of SB in rat-cultured cortical neurons and in human clonal epithelial cells (HeLa cells) using cell death assays and the fluorescent measurements of intracellular Ca2+ concentration ([Ca2+](i)) and mitochondrial transmembrane potential (Delta psi(m)). We found that SB induced cell death in a dose-dependent manner. Even 0.1% (w/v) SB, a concentration typically added to soft drinks, produced a significant decrease in cell viability in rat cortical and HeLa cells. We also found that SB caused [Ca2+](i) and Delta psi(m) increases in a dose-dependent manner. Based on our results, more caution and further cellular studies are required with respect to the use of SB as a food preservative. English WILEY BLOOD-BRAIN-BARRIER MITOCHONDRIAL-MEMBRANE OXIDATIVE STRESS APOPTOSIS DEATH RAT TOXICITY DAMAGE DRUGS YEAST SODIUM BENZOATE-MEDIATED CYTOTOXICITY IN MAMMALIAN CELLS Article 10.1111/j.1745-4514.2010.00432.x 1 JOURNAL OF FOOD BIOCHEMISTRY, v.35, no.4, pp.1034 - 1046 JOURNAL OF FOOD BIOCHEMISTRY 35 4 1034 1046 scie scopus 000297475700002 2-s2.0-79960958696 Biochemistry & Molecular Biology Food Science & Technology Biochemistry & Molecular Biology Food Science & Technology Article BLOOD-BRAIN-BARRIER MITOCHONDRIAL-MEMBRANE OXIDATIVE STRESS APOPTOSIS DEATH RAT TOXICITY DAMAGE DRUGS YEAST