Choi, Seung-Hye Kwon, Ick Chan Hwang, Kwang Yeon Kim, In-San Ahn, Hyung Jun 2024-01-20T16:33:29Z 2024-01-20T16:33:29Z 2021-09-05 2011-08 1525-7797 https://pubs.kist.re.kr/handle/201004/130141 Protein cages have the potential to serve as biomaterials for the targeted therapeutic and imaging systems. As an effort to exploit small heat shock protein (Hip) cages as multifunctional biomaterials, we demonstrate that chemically and genetically modified Hsp cages permeate the cells via cancer cell binding and subsequent endocytic internalization and can image caspase activity in the live cells. Moreover, we,report here that these functional Hsp cages can be specifically accumulated to tumor tissues of tumor-bearing mice when administered intravenously through the lateral tail vein. These tumor-targeting Properties could be explained by the prolonged in vivo circulation and enhanced permeability and retention (EPR) effect as well as the ligand-mediated binding to cancer cells. Furthermore, when combined with the caspase sensing ability, our Hsp cage allows us to monitor the therapeutic evaluation after anticancer drug treatment by imaging the caspase activity within tumors. Therefore, we demonstrate that the Hsp cages have multifunctional scaffolds amenable to genetic and chemical modifications without loss of the cagelike architecture and can be exploited as biomedical materials including drug or imaging agent carriers. English AMER CHEMICAL SOC DRUG-DELIVERY COATED PIT APOPTOSIS PATHWAYS Small Heat Shock Protein as a Multifunctional Scaffold: Integrated Tumor Targeting and Caspase Imaging within a Single Cage Article 10.1021/bm200743g 1 BIOMACROMOLECULES, v.12, no.8, pp.3099 - 3106 BIOMACROMOLECULES 12 8 3099 3106 scie scopus 000293488200030 2-s2.0-80051482370 Biochemistry & Molecular Biology Chemistry, Organic Polymer Science Biochemistry & Molecular Biology Chemistry Polymer Science Article DRUG-DELIVERY COATED PIT APOPTOSIS PATHWAYS 열충격단백질 다기능성 구조물 암 표적성 세포사멸 분자영상