Moon, Ju-Yeon Kim, Kwang Joon Moon, Myeong Hee Chung, Bong Chul Choi, Man Ho 2024-01-20T16:33:39Z 2024-01-20T16:33:39Z 2021-09-04 2011-08 0022-2275 https://pubs.kist.re.kr/handle/201004/130150 Estrogen metabolites play important roles in the development of female-related disorders and homeostasis of the bone. To improve detectability, a validated gas chromatography-mass spectrometry (GC-MS) method was conducted with two-phase extractive ethoxycarbonlyation (EOC) and subsequent pentafluoropropionyl (PFP) derivatization was introduced. The resulting samples were separated through a high-temperature MXT-1 column within an 8 min run and were detected in the selected ion monitoring (SIM) mode. The optimized analytical conditions led to good separation with a symmetric peak shape for 19 estrogens as their EOC-PFP derivatives. The limit of quantification (LOQ) was from 0.02 to similar to 0.1 ng/ml for most estrogens analyzed, except for 2-hydroxyestriol (0.5 ng/ml). The devised method was found to be linear (r(2) > 0.995) in the range from the LOQ to 40 ng/ml, whereas the precision (% CV) and accuracy (% bias) ranged from 1.4 to 10.5% and from 91.4 to 108.5%, respectively. The good sensitivity and selectivity of this method even allowed quantification of the estrogen metabolites in urine samples obtained from the postmenopausal female patients with osteoporosis. The present technique can be useful for clinical diagnosis as well as to better understand the pathogenesis of estrogen-related disorders in low-level quantification.-Moon, J-Y., K. J. Kim, M. H. Moon, B. C. Chung, and M. H. Choi. A novel GC-MS method in urinary estrogen analysis from postmenopausal women with osteoporosis. J. Lipid Res. 2011. 52: 1595-1603. English AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC CHROMATOGRAPHY-MASS SPECTROMETRY GAS-CHROMATOGRAPHY BREAST-CANCER METABOLITES ESTRADIOL DERIVATIZATION SERUM RISK 17-BETA-ESTRADIOL REPRODUCIBILITY A novel GC-MS method in urinary estrogen analysis from postmenopausal women with osteoporosis Article 10.1194/jlr.D016113 1 JOURNAL OF LIPID RESEARCH, v.52, no.8, pp.1595 - 1603 JOURNAL OF LIPID RESEARCH 52 8 1595 1603 scie scopus 000292763200018 2-s2.0-79960672388 Biochemistry & Molecular Biology Biochemistry & Molecular Biology Article CHROMATOGRAPHY-MASS SPECTROMETRY GAS-CHROMATOGRAPHY BREAST-CANCER METABOLITES ESTRADIOL DERIVATIZATION SERUM RISK 17-BETA-ESTRADIOL REPRODUCIBILITY steroid profiling ethoxycarbonylation hormones