Kim, Yeon Seok Jurng, Jongsoo 2024-01-20T16:33:51Z 2024-01-20T16:33:51Z 2021-09-02 2011-08 0003-2654 https://pubs.kist.re.kr/handle/201004/130161 We developed a homogeneous fluorescence assay for multiplex detection based on the target induced conformational change of DNA aptamers. DNA aptamers were immobilized on quantum dots (QDs), and QDs conjugated ssDNA was adsorbed on the surface of gold nanoparticles (AuNPs) by electrostatic interaction between uncoiled ssDNA and the AuNPs. Subsequently the fluorescence of QDs was effectively quenched by the AuNPs due to fluorescence resonance energy transfer (FRET) of QDs to AuNPs. In the presence of targets, the QDs conjugated aptamers were detached from AuNPs by target induced conformational change of aptamers, consequently the fluorescence of the QDs was recovered proportional to the target concentration. In this study, three different QD/aptamer conjugates were used for multiplex detection of mercury ions, adenosine and potassium ions. In a control experiment, all of the three targets were simultaneously detected with high selectivity. English Royal Society of Chemistry COLORIMETRIC DETECTION QUANTUM DOTS SELECTIVE DETECTION BIOLOGICAL-FLUIDS HG2+ PROBES ASSAY IONS APTAMERS DIAGNOSTICS Gold nanoparticle-based homogeneous fluorescent aptasensor for multiplex detection Article 10.1039/c1an15261k 1 Analyst, v.136, no.18, pp.3720 - 3724 Analyst 136 18 3720 3724 scie scopus 000294123300022 2-s2.0-80052219720 Chemistry, Analytical Chemistry Article COLORIMETRIC DETECTION QUANTUM DOTS SELECTIVE DETECTION BIOLOGICAL-FLUIDS HG2+ PROBES ASSAY IONS APTAMERS DIAGNOSTICS