Kim, Sang Min Kang, Kyungsu Jho, Eun Hye Jung, Yu-Jin Nho, Chu Won Um, Byung-Hun Pan, Cheol-Ho 2024-01-20T16:34:38Z 2024-01-20T16:34:38Z 2021-09-05 2011-07 0951-418X https://pubs.kist.re.kr/handle/201004/130196 The aerial parts of Lespedeza cuneata G. Don, perennial legume native to Eastern Asia, have been used therapeutically in traditional Asian medicine to protect the function of liver, kidneys and lungs. However, little is known about the pharmaceutical effect of extracts from this plant. In the present study, the aerial parts of L. cuneata were used to prepare an ethanol extract, which was then tested for hepatoprotective effects against injury by tert-butyl hyperoxide (t-BHP). At a dose of 20 mu g/mL, the ethanol extract significantly protected HepG2 cells against the cytotoxicity of t-BHP. Further fractionation of the extract with ethyl acetate allowed the isolation of five flavonoid compounds that were structurally identified by H-1 and C-13 NMR spectroscopy as isovitexin, hirsutrin, trifolin, avicularin and quercetin. Hirsutrin, avicularin and quercetin (10 mu M) showed clear hepatoprotective activity against injury by t-BHP in HepG2 cells, whereas isovitexin and trifolin showed no protective effects. The observed hepatoprotective effect of the investigated compounds showed a high correlation with radical scavenging activity, which followed the structure-activity relationships of the flavonoid aglycones. Copyright (C) 2011 John Wiley & Sons, Ltd. English WILEY C-GLYCOSYLFLAVONES HEART-DISEASE CONSTITUENTS METABOLISM ANTIOXIDANTS LEAVES PLANTS HEPG2 Hepatoprotective Effect of Flavonoid Glycosides from Lespedeza cuneata against Oxidative Stress Induced by tert-Butyl Hyperoxide Article 10.1002/ptr.3387 1 PHYTOTHERAPY RESEARCH, v.25, no.7, pp.1011 - 1017 PHYTOTHERAPY RESEARCH 25 7 1011 1017 scie scopus 000293031400008 2-s2.0-84860420552 Chemistry, Medicinal Pharmacology & Pharmacy Pharmacology & Pharmacy Article C-GLYCOSYLFLAVONES HEART-DISEASE CONSTITUENTS METABOLISM ANTIOXIDANTS LEAVES PLANTS HEPG2 avicularin hepatoprotective effect hirsutrin Lespedeza cuneata quercetin radical scavenging activity