Jin, Ming J. Jin, Changbae Kim, Jin Y. In, Moon K. Kwon, Oh S. Yoo, Hye H. 2024-01-20T16:34:51Z 2024-01-20T16:34:51Z 2021-09-05 2011-07 0022-1198 https://pubs.kist.re.kr/handle/201004/130207 5-Methoxy-N,N-diisopropyltryptamine (5-MeO-DIPT) is a designer hallucinogen derived from tryptamine and is reportedly abused and involved in criminal activities. For the detection of 5-MeO-DIPT use, a liquid chromatography-tandem mass spectrometric method for 5-MeO-DIPT and its metabolites, 5-hydroxy-N,N-diisopropyltryptamine (5-OH-DIPT) and 5-methoxy-N,N-isopropyltryptamine (5-MeO-IPT) was developed and validated in rat urine. The urine samples were pretreated by protein precipitation with acetonitrile and introduced into a BDS HYPERSIL C-18 column (50 x 2.0 mm, 5 mu m) for chromatographic separation. Mobile phases consisted of methanol, water, and 1% formic acid, and gradient elution was used at a flow rate of 0.2 mL/min. For the MS detection, multiple-reaction monitoring analysis was adopted. The linear range was 0.01-10 mu g/mL, and the lower limit of quantification was 10 ng/mL for all analytes. The intra- and interday accuracies and precisions met the criteria (< 15%). The developed method was successfully applied to the drug-treated rat urine. English WILEY A Quantitative Method for Simultaneous Determination of 5-Methoxy-N,N-Diisopropyltryptamine and its Metabolites in Urine Using Liquid Chromatography-Electrospray Ionization-Tandem Mass Spectrometry Article 10.1111/j.1556-4029.2011.01753.x 1 JOURNAL OF FORENSIC SCIENCES, v.56, no.4, pp.1044 - 1048 JOURNAL OF FORENSIC SCIENCES 56 4 1044 1048 N scie scopus 000292448400034 2-s2.0-79960060046 Medicine, Legal Legal Medicine Article LIVER-MICROSOMES N,N-DIISOPROPYLTRYPTAMINE FOXY forensic science 5-MeO-DIPT 5-OH-DIPT 5-MeO-IPT urine LC/MS/MS