Jung, Hyun-Jin Kim, Sun Ju Lee, Won-Yong Chung, Bong Chul Choi, Man Ho 2024-01-20T17:02:39Z 2024-01-20T17:02:39Z 2021-09-04 2011-05-15 0951-4198 https://pubs.kist.re.kr/handle/201004/130352 A method of steroid profiling, including androgens, progestins, corticoids and sterols, was developed to evaluate the concentrations of steroids as well as the activities of the enzymes responsible for steroidogenesis in hair by gas chromatography/mass spectrometry. The extraction efficiencies of steroids from the hair matrix were improved by ultrasonication for 1 h at 50 degrees C. The overall recoveries ranged from 71 to 132%, with a limit of quantification for all analytes ranging from 1 to 50 ng/g. The devised method was used to identify the metabolic changes for both male-pattern baldness (MPB) and the drug efficiency of dutasteride, which inhibits 5 alpha-reductase. Increased dihydrotestosterone levels and the dihydrotestosterone/testosterone (DHT/T) ratio, which is responsible for the 5 alpha-reductase activity, were observed in the MPB patients. A dutasteride treatment resulted in decreases in the DHT and 5 alpha-androstanedione concentrations and DHT/T ratio in the hair samples. Hair steroid profiling reflects the sebaceous status in the scalp and may be useful for monitoring the metabolic responses to both the disease and drug actions. Copyright (C) 2011 John Wiley & Sons, Ltd. English WILEY-BLACKWELL TANDEM MASS-SPECTROMETRY BENIGN PROSTATIC HYPERPLASIA MALE-PATTERN BALDNESS ANDROGENETIC ALOPECIA BIOLOGICAL MATRICES DUAL INHIBITOR DERMAL PAPILLA ALCOHOL-ABUSE MEN PLASMA Gas chromatography/mass spectrometry based hair steroid profiling may reveal pathogenesis in hair follicles of the scalp Article 10.1002/rcm.4975 1 RAPID COMMUNICATIONS IN MASS SPECTROMETRY, v.25, no.9, pp.1184 - 1192 RAPID COMMUNICATIONS IN MASS SPECTROMETRY 25 9 1184 1192 scie scopus 000290452600008 2-s2.0-79954439929 Biochemical Research Methods Chemistry, Analytical Spectroscopy Biochemistry & Molecular Biology Chemistry Spectroscopy Article TANDEM MASS-SPECTROMETRY BENIGN PROSTATIC HYPERPLASIA MALE-PATTERN BALDNESS ANDROGENETIC ALOPECIA BIOLOGICAL MATRICES DUAL INHIBITOR DERMAL PAPILLA ALCOHOL-ABUSE MEN PLASMA GC-MS hair steroid baldness dutasteride