Shehzad, Omer Ha, In Jin Park, Youmie Ha, Young Wan Kim, Yeong Shik 2024-01-20T17:02:57Z 2024-01-20T17:02:57Z 2022-01-25 2011-05 1615-9306 https://pubs.kist.re.kr/handle/201004/130365 Ginsenosides exhibit diverse biological activities and are major well-known components isolated from the radix of Panax ginseng C. A. Meyer. In the present work, a rapid and facile method for the separation and purification of eight ginsenosides from P. ginseng by highspeed counter-current chromatography coupled with evaporative light scattering detector (HSCCC-ELSD) was successfully developed. The crude samples for HSCCC separation were first purified from ginseng extract using a macroporous resin; the extract was loaded onto a Diaion-HP20 column and fractionated by methanol and water gradient elution. The ginsenosides-protopanaxadiol (PPD) and protopanaxatriol (PPT) fractions were subsequently eluted with 65 and 80% methanol and water gradient elution, respectively. Furthermore, these two fractions were separated by HSCCC-ELSD. The two-phase solvent system used for separation was composed of chloroform/methanol/water/isopropanol at a volume ratio of 4:3:2:1. Each fraction obtained was collected and dried, yielding the following eight ginsenosides: Rg(1), Re, Rf, Rh(1), Rb(1), Rc Rb(2) and Rd. The purity of these ginsenosides was greater than 97% as assessed by HPLC-ELSD, and their structures were characterized by electrospray-ionization mass spectrometry (ESI-MS) and nuclear magnetic resonance spectroscopy. This is the first report regarding the separation of the ginsenosides Rh(1), Rb(2) and Rc from P. ginseng by HSCCC. English WILEY-BLACKWELL Development of a rapid and convenient method to separate eight ginsenosides from Panax ginseng by high-speed countercurrent chromatography coupled with evaporative light scattering detection Article 10.1002/jssc.201000932 1 JOURNAL OF SEPARATION SCIENCE, v.34, no.10, pp.1116 - 1122 JOURNAL OF SEPARATION SCIENCE 34 10 1116 1122 N scie scopus 000290635400004 2-s2.0-79955688677 Chemistry, Analytical Chemistry Article PERFORMANCE LIQUID-CHROMATOGRAPHY TRADITIONAL CHINESE MEDICINE DAMMARANE-SAPONINS RB-1 NOTOGINSENG GLYCOSIDES ROOT Ginsenosides High-speed counter-current chromatography Panax ginseng Protopanaxadiol Protopanaxatriol