Lee, Jin Yong Kim, Byoung Chan Chang, Kwan Jong Ahn, Joo-Myung Ryu, Jee-Hoon Chang, Hyo-Ihl Gu, Man Bock 2024-01-20T17:03:24Z 2024-01-20T17:03:24Z 2021-09-02 2011-05 0273-2289 https://pubs.kist.re.kr/handle/201004/130388 In this study, we present the successful detection of food-borne pathogens using randomly selected non-sequenced genomic DNA probes-based DNA microarray chips. Three food-borne pathogens, Staphylococcus aureus, Salmonella enterica subsp. enterica serovar Typhimurium (S. Typhimurium), and Bacillus cereus, were subjected for the preparation of the DNA microarray probes. Initially, about 50 DNA probes selected randomly from non-sequenced genomic DNA of each pathogen were prepared by using a set of restriction enzyme pairs. The proto-type of DNA microarray chip for detecting three different pathogens simultaneously was fabricated by using those DNA probes prepared for each pathogen. This proto-type DNA microarray has been tested with three target pathogens and additional seven bacteria, and successfully verified with a few cross-hybridized probes. After this primary verification of the DNA microarray hybridization, this proto-type DNA microarray chip was redesigned and successfully optimized by eliminating a few cross-hybridized probes. The specificity of this redesigned DNA microarray chip to each pathogen was confirmed without any serious cross-hybridizations, and its multiplexing capability in its pathogen detection was found to be possible. This randomly selected non-sequenced genomic DNA probes-based DNA microarray was successfully proved to be the high-throughput simultaneous detection chip for the detection of food-borne pathogens, without knowing the exact sequence information of the target bacteria. This could be the first fabrication of DNA microarray chip for the simultaneous detection of different kinds of food-borne pathogens. English HUMANA PRESS INC GRADIENT GEL-ELECTROPHORESIS FOODBORNE PATHOGENS ESCHERICHIA-COLI IDENTIFICATION BACTERIA PCR HYBRIDIZATION MILK DGGE A Subtractively Optimized DNA Microarray Using Non-sequenced Genomic Probes for the Detection of Food-Borne Pathogens Article 10.1007/s12010-010-9126-6 1 APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, v.164, no.2, pp.183 - 193 APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY 164 2 183 193 scie scopus 000288898500006 2-s2.0-79956344539 Biochemistry & Molecular Biology Biotechnology & Applied Microbiology Biochemistry & Molecular Biology Biotechnology & Applied Microbiology Article GRADIENT GEL-ELECTROPHORESIS FOODBORNE PATHOGENS ESCHERICHIA-COLI IDENTIFICATION BACTERIA PCR HYBRIDIZATION MILK DGGE Food-borne pathogen DNA microarray chip Non-sequenced genomic DNA probes Cross-hybridization Multiplexed detection