Lee, Soyoung Choi, Jungil Shin, Sangwon Im, Yeon-Min Song, Jonghan Kang, Sang Soo Nam, Tae-Hyun Webster, Thomas J. Kim, Sang-Hyun Khang, Dongwoo 2024-01-20T17:03:58Z 2024-01-20T17:03:58Z 2021-09-02 2011-05 1742-7061 https://pubs.kist.re.kr/handle/201004/130418 The immunotoxicity of implanted nanostructured titanium is a paramount issue for vascular, dental and orthopedic applications. However, it has been unclear whether implanted surface nanostructures can inhibit or aggrevate inflammatory responses. Herein, macrophage activation, as evidence of migration, on transparent flat and nanostructured titanium correlated with pro-inflammatory protein synthesis and cytokine release. Through the real-time monitoring of initial cytoskeleton variations, this study identified that macrophage movement was restricted on nanostructured titanium compared to flat titanium surfaces. Furthermore, nanostructured titanium elicited secretion of fewer pro-inflammatory enzyme molecules and cytokines, as well as reduced nitric oxide production. All results collectively indicated that initial macrophage activation can be mitigated by nanoscale surface topography alone, without modification of surface chemistry or stiffness. (C) 2011 Acta Materialia Inc. Published by Elsevier Ltd All rights reserved English ELSEVIER SCI LTD NITRIC-OXIDE SYNTHASE SURFACE-TOPOGRAPHY EXPRESSION ADHESION MECHANISMS CYTOKINES RESPONSES COLLAGEN CELLS Analysis on migration and activation of live macrophages on transparent flat and nanostructured titanium Article 10.1016/j.actbio.2011.01.006 1 ACTA BIOMATERIALIA, v.7, no.5, pp.2337 - 2344 ACTA BIOMATERIALIA 7 5 2337 2344 scie scopus 000290649500045 2-s2.0-79953862226 Engineering, Biomedical Materials Science, Biomaterials Engineering Materials Science Article NITRIC-OXIDE SYNTHASE SURFACE-TOPOGRAPHY EXPRESSION ADHESION MECHANISMS CYTOKINES RESPONSES COLLAGEN CELLS Immunotoxicity Implant Macrophage Titanium Live cell migration