Hong, Sung-Woon Yang, Jeong-hwa Joh, Eun-Ha Kim, Hyoung Ja Kim, Dong-Hyun 2024-01-20T17:04:34Z 2024-01-20T17:04:34Z 2021-09-02 2011-04-12 0378-8741 https://pubs.kist.re.kr/handle/201004/130447 Ethnopharmacological relevance: Gynostemma pentaphyllum (Thunb.) Makino (GP, family Cucurbitaceae), which contains dammarane saponins as its main constituents, is used in China, Japan, and Korea as a traditional medicine to treat cancer, obesity, arteriosclerosis, asthma and senility. Aim of the study: To investigate the memory-enhancing effects of GP, Gypenoside TN-2 (TN-2) was isolated by activity-guided fractionation and administered to scopolamine-induced memory-deficient mice. Materials and methods: The memory-enhancing effects of TN-2 were evaluated using passive avoidance. Y-maze, and Morris water maze tests, and the protein expressions of brain-derived neurotrophic factor (BDNF), cAMP element binding protein (CREB), and p-CREB were determined by immunoblotting. Results: TN-2 inhibited memory and learning deficits in scopolamine treated mice in the passive avoidance test. TN-2 (10, 20, and 40 mg/kg, p.o.) significantly inhibited memory and learning deficits in the passive avoidance test by 40%, 96% and 78%, respectively, and exhibited significant memory-enhancing effects on the Y-maze test and the Morris water maze test. TN-2 also markedly increased BNDF expression and activated the transcription factor CREB in the hippocampi of scopolamine-treated mice. Conclusions: TN-2 may ameliorate memory and learning deficits by activating the CREB-BDNF pathway. (C) 2011 Elsevier Ireland Ltd. All rights reserved. English ELSEVIER IRELAND LTD LONG-TERM-MEMORY GYNOSTEMMA-PENTAPHYLLUM BDNF Gypenoside TN-2 ameliorates scopolamine-induced learning deficit in mice Article 10.1016/j.jep.2011.02.002 1 JOURNAL OF ETHNOPHARMACOLOGY, v.134, no.3, pp.1010 - 1013 JOURNAL OF ETHNOPHARMACOLOGY 134 3 1010 1013 scie scopus 000289866500058 2-s2.0-79954423234 Plant Sciences Chemistry, Medicinal Integrative & Complementary Medicine Pharmacology & Pharmacy Plant Sciences Pharmacology & Pharmacy Integrative & Complementary Medicine Article LONG-TERM-MEMORY GYNOSTEMMA-PENTAPHYLLUM BDNF Gynostemma pentaphyllum (Thunb.) Makino Gypenoside TN-2 Memory Scopolamine Brain-derived neurotrophic factor