Park, Min Jose, Joachim Thoemmes, Sarah Kim, Jo-Il Kang, Min-Jung Pyun, Jae-Chul 2024-01-20T17:04:39Z 2024-01-20T17:04:39Z 2021-09-02 2011-04-07 0141-0229 https://pubs.kist.re.kr/handle/201004/130450 Streptavidin was expressed on the outer membrane of E. coli as a recombinant fusion protein with an autotransporter domain called AIDA-I (adhesin involved in diffuse adherence) using autodisplay technology. The autodisplay of streptavidin was confirmed by SOS-PAGE of the outer membrane proteins, and the number of autodisplayed streptavidin molecules on a single E. coli cell was evaluated with densitometric analysis. The biotin-binding activity of the autodisplayed streptavidin was estimated after treatment with fluorescently labeled biotin by fluorescence microscopy and flow cytometry. The biotin-binding activity of the E. coli with autodisplayed streptavidin was compared with the activity of streptavidin immobilized on magnetic beads. Finally, the outer membrane presenting autodisplayed streptavidin was isolated and layered on a 96-well microplate for an immunoassay. (C) 2010 Elsevier Inc. All rights reserved. English ELSEVIER SCIENCE INC BACTERIAL SURFACE DISPLAY ESCHERICHIA-COLI PROTEIN-A ANTIBODY IMMOBILIZATION OUTER-MEMBRANE AUTOTRANSPORTER PATHWAY RECOMBINANT PROTEINS Z-DOMAIN AIDA-I AVIDIN Autodisplay of streptavidin Article 10.1016/j.enzmictec.2010.12.006 1 ENZYME AND MICROBIAL TECHNOLOGY, v.48, no.4-5, pp.307 - 311 ENZYME AND MICROBIAL TECHNOLOGY 48 4-5 307 311 scie scopus 000289930900001 2-s2.0-79953060571 Biotechnology & Applied Microbiology Biotechnology & Applied Microbiology Article BACTERIAL SURFACE DISPLAY ESCHERICHIA-COLI PROTEIN-A ANTIBODY IMMOBILIZATION OUTER-MEMBRANE AUTOTRANSPORTER PATHWAY RECOMBINANT PROTEINS Z-DOMAIN AIDA-I AVIDIN Autodisplay Streptavidin E. coli Immunoassay