Kim, Kyung-Woo Kim, Byung Chan Lee, Hye Jin Kim, Jungbae Oh, Min-Kyu 2024-01-20T17:05:08Z 2024-01-20T17:05:08Z 2021-09-02 2011-04 1040-0397 https://pubs.kist.re.kr/handle/201004/130475 Enzyme-based Boolean logic gates were developed by using highly stable enzyme precipitate coatings (EPCs) on carbon nanotubes (CNTs). EPCs of three enzymes, glucose oxidase (GOx), horseradish peroxidase (HRP), and invertase (INV), were prepared in a three-step process that consists of covalent enzyme attachment, enzyme precipitation, and enzyme crosslinking. The AND logic gate was formulated based on an ON output signal in response to the combined inputs of glucose and dye. The OR logic gate was based on the ON output signal of glucose oxidation in the presence of EPC-GOx and EPC-INV with one of the two inputs, either glucose or sucrose. The use of EPCs will provide an effective tool for the development of sensitive and repeatedly-usable Boolean logic gates with a long lifetime, which can be employed in various biosignaling systems. English WILEY-V C H VERLAG GMBH MOLECULAR LOGIC BIOFUEL CELLS NANOPARTICLES STABILITY SYSTEM SIGNALS Enzyme Logic Gates Based on Enzyme-Coated Carbon Nanotubes Article 10.1002/elan.201000634 1 ELECTROANALYSIS, v.23, no.4, pp.980 - 986 ELECTROANALYSIS 23 4 980 986 scie scopus 000289264900024 2-s2.0-79953267399 Chemistry, Analytical Electrochemistry Chemistry Electrochemistry Article MOLECULAR LOGIC BIOFUEL CELLS NANOPARTICLES STABILITY SYSTEM SIGNALS Enzyme precipitate coatings Boolean logic gates Carbon nanotubes Glucose oxidase Horseradish peroxidase Invertase