Bae, Nayoung Ahn, Taekwon Chung, Sungkwon Oh, Myung Sook Ko, Hyeonseok Oh, Hyungun Park, Gunhyuk Yang, Hyun Ok 2024-01-20T17:30:46Z 2024-01-20T17:30:46Z 2021-09-02 2011-03-24 0378-8741 https://pubs.kist.re.kr/handle/201004/130510 Aim of study: Modified Yeoldahanso-tang (MYH) is a Korean herbal formula, containing 10 herbs: Pueraria lobata (Willd.) Ohwi. Angelica tenuissima Nakai, Scutellaria baicalensis Georgi, Platycodon grandiflorum (Jacq), Angelicae Dahurica, Cimicifuga heracleifolia Kom, Raphanus sativa L, Polygala tenuifolia (Willd.), Acorus gramineus Soland. and Dimoccupus longan Lour. The constitutive ratio of the ten herbs is at 6:4:2:1:2:2:2:4:6:6 in dry weight. MYH has been used to treat amnesia, hypochondria and dementia in Korea. In this study, we explored the possibility of using MYH in the prevention and treatment of Parkinson's disease (PD). Specifically, we made an effort to demonstrate the neuroprotective effects of MYH using experimental methods similar to those used in a recent study of PD. Materials and methods: 1-Methyl-4-phenylpyridinium (MPP+) (400 p,M) was used to induce cytotoxicity in NGF (nerve growth factor)-differentiated PC12 cells. Cell viability was measured using a mu assay. Induction of autophagy by MYH in NGF-differentiated PC12 cells was measured using an immunoblotting assay with LC3 and beclin 1 antibodies. The proteasomal inhibitor lactacystin (10 p,M) was used to cause UPS dysfunction in NGF-differentiated PC12 cells. Clearance of aggregated proteins by MYH was measured using an immunoblotting assay with an ubiquitin antibody. 1-Methyl-4-phenyl-1,2,3,6-tetrahydrophenylpyridine (MPTP) (20 mg/kg, 4 times i.p.) caused substantia nigra injuries in C57BL/6 mice. Dopamine (DA) neurons were identified using a tyrosine hydroxylase-immunohistochemistry (TH-IHC) assay with a rabbit anti-TH antibody. Results: Our findings indicate that MYH provides protection against MPP+-induced injury in NGF-differentiated PC12 cell. And MYH provides neuroprotection against lactacystin-induced NGF-differentiated PC12 cell death, which effect is partially mediated by autophagy enhancement through enhanced degradation of aggregated proteins. Additionally, in a C57BL/6 mice model with MFTP-induced substantia nigra injuries, MYH inhibits both the loss of TH-positive neurons in the substantia nigra pars compacta (SNpc) and the reduction of the optical density of TH-IR fibers in the striatum (ST). Conclusions: All of our results indicate that MYH treatment has neuroprotective effects that are partially mediated by autophagy enhancement. MYH may be a promising herbal formula for the prevention and treatment of neurodegenerative diseases, especially PD. (C) 2010 Elsevier Ireland Ltd. All rights reserved. English ELSEVIER IRELAND LTD CHAPERONE-MEDIATED AUTOPHAGY ISOLATED RAT HEPATOCYTES TYROSINE-HYDROXYLASE ALPHA-SYNUCLEIN PROTEASOME NEURODEGENERATION DEGRADATION INHIBITORS NEUROTOXICITY MUTATIONS The neuroprotective effect of modified Yeoldahanso-tang via autophagy enhancement in models of Parkinson's disease Article 10.1016/j.jep.2010.12.016 1 JOURNAL OF ETHNOPHARMACOLOGY, v.134, no.2, pp.313 - 322 JOURNAL OF ETHNOPHARMACOLOGY 134 2 313 322 scie scopus 000289329500017 2-s2.0-79952696618 Plant Sciences Chemistry, Medicinal Integrative & Complementary Medicine Pharmacology & Pharmacy Plant Sciences Pharmacology & Pharmacy Integrative & Complementary Medicine Article CHAPERONE-MEDIATED AUTOPHAGY ISOLATED RAT HEPATOCYTES TYROSINE-HYDROXYLASE ALPHA-SYNUCLEIN PROTEASOME NEURODEGENERATION DEGRADATION INHIBITORS NEUROTOXICITY MUTATIONS Modified Youldahanso-tang (MYH) Neuroprotection Autophagy Parkinson&apos s disease 1-Methyl-4-phenyl-1,2,3,6-tetrahydrophenylpyridine (MPTP) Lactacystin