Sarma, Sailendra Nath Kim, Youn-Jung Song, Mee Ryu, Jae-Chun 2024-01-20T17:33:09Z 2024-01-20T17:33:09Z 2021-09-02 2011-02-27 0300-483X https://pubs.kist.re.kr/handle/201004/130628 Whereas benzene (BZ) is a well-known human carcinogen, toluene (TOL) and o-xylene (o-XY) are not; however, all three compounds are important environmental pollutants. Although BZ, TOL, and o-XY have been shown to induce apoptosis in vitro, their mechanism of toxicity remains unclear. In this study, we sought to identify the apoptotic pathway(s) activated by BZ, TOL, and o-XY in human HL-60 promyelocytic leukemia cells. Cell cycle analysis by propidium iodide (PI) staining and flow cytometric analyses of Annexin V/PI double-stained cells revealed similar patterns of apoptosis following BZ, TOL, and o-XY exposure. Though reactive oxygen species (ROS) production contributes significantly to BZ metabolite-induced apoptotic cell death, we hypothesized that BZ, TOL, and o-XY can themselves trigger ROS production, leading to the activation of apoptotic signaling. Dose-dependent increases in ROS production and significant tail moments were observed in HL-60 cells exposed to all three compounds. Real-time RT-PCR revealed increased HMOX1 and Noxa expression in BZ-, TOL-, and o-XY-treated HL-60 cells, confirming the results of previous microarray analyses. Similar expression profiles were found in human K562 ery-thromyeloblastoid leukemia cells and human U937 leukemic monocyte lymphoma cells. Pretreatment with the ROS scavenger N-acetyl cysteine decreased the effects of exposure to BZ, TOL, and o-XY. In summary, this study provides useful insights into the mechanism of BZ-. TOL-, and o-XY-induced apoptosis in leukemia cells. (C) 2010 Elsevier Ireland Ltd. All rights reserved. English ELSEVIER IRELAND LTD VOLATILE ORGANIC-COMPOUNDS HEME OXYGENASE-1 OXIDATIVE STRESS GENE-EXPRESSION MELANOMA-CELLS DEATH EXPOSURE CANCER GENERATION INHIBITOR Induction of apoptosis in human leukemia cells through the production of reactive oxygen species and activation of HMOX1 and Noxa by benzene, toluene, and o-xylene Article 10.1016/j.tox.2010.11.017 1 TOXICOLOGY, v.280, no.3, pp.109 - 117 TOXICOLOGY 280 3 109 117 scie scopus 000287436100005 2-s2.0-79151470137 Pharmacology & Pharmacy Toxicology Pharmacology & Pharmacy Toxicology Article VOLATILE ORGANIC-COMPOUNDS HEME OXYGENASE-1 OXIDATIVE STRESS GENE-EXPRESSION MELANOMA-CELLS DEATH EXPOSURE CANCER GENERATION INHIBITOR Benzene Toluene o-Xylene Apoptosis HMOX1 Noxa