Lee, Seulki Choi, Ki Young Chung, Hyunjin Ryu, Ju Hee Lee, Aeju Koo, Heebeom Youn, In-Chan Park, Jae Hyung Kim, In-San Kim, Sang Yoon Chen, Xiaoyuan Jeong, Seo Young Kwon, Ick Chan Kim, Kwangmeyung Choi, Kuiwon 2024-01-20T17:34:09Z 2024-01-20T17:34:09Z 2021-09-02 2011-02 1043-1802 https://pubs.kist.re.kr/handle/201004/130677 We report a new apoptosis nanoprobe (Apo-NP) designed on the basis of a polymer nanoparticle platform. This simple one-step technique is capable of boosting fluorescence signals upon apoptosis in living cells, enabling real-time imaging of apoptosis in single cells and in vivo. The Apo-NP efficiently delivers chemically labeled, dual-quenched caspase-3-sensitive fluorogenic peptides into cells, allowing caspase-3-dependent strong fluorescence amplification to be imaged in apoptotic cells in real-time and at high resolution. The design platform of the Apo-NP is flexible and can be fine-tuned for a wide array of applications such as identification of caspase-related apoptosis in pathologies and for monitoring therapeutic efficacy of apoptotic drugs in cancer treatment. English AMER CHEMICAL SOC PROBES Real Time, High Resolution Video Imaging of Apoptosis in Single Cells with a Polymeric Nanoprobe Article 10.1021/bc1004119 1 BIOCONJUGATE CHEMISTRY, v.22, no.2, pp.125 - 131 BIOCONJUGATE CHEMISTRY 22 2 125 131 scie scopus 000287297000003 2-s2.0-79951701642 Biochemical Research Methods Biochemistry & Molecular Biology Chemistry, Multidisciplinary Chemistry, Organic Biochemistry & Molecular Biology Chemistry Article PROBES