Kim, Nam Hyun Kim, Su-Nam Kim, Yong Kee 2024-01-20T17:34:58Z 2024-01-20T17:34:58Z 2021-09-02 2011-01-28 0006-291X https://pubs.kist.re.kr/handle/201004/130716 In this study, we investigate the molecular mechanism by which histone deacetylase (HDAC) inhibitors exert anti-invasiveness effect against prostate cancer cells. We first evaluate the growth inhibition effect of HDAC inhibitors in prostate cancer cells, which is accompanied by induction of p21(WAF1) expression and accumulation of acetylated histones. And we found that the migration and invasion of prostate cancer cells is strongly inhibited by treatment with HDAC inhibitors. In parallel, E-cadherin level is highly up-regulated in HDAC inhibitor-treated prostate cancer cells. And siRNA knockdown of E-cadherin significantly diminishes the anti-invasion effect of HDAC inhibitors, indicating that E-cadherin overexpression is one of possible mechanism for anti-invasion effect of HDAC inhibitors. Furthermore, specific downregulation of HDAC1, but not HDAC2, causes E-cadherin expression and subsequent inhibition of cell motility and invasion. Collectively, our data demonstrate that HDAC1 is a major repressive enzyme for E-cadherin expression as well as HDAC inhibitor-mediated anti-invasiveness. (C) 2010 Elsevier Inc. All rights reserved. English ACADEMIC PRESS INC ELSEVIER SCIENCE HISTONE DEACETYLASE INHIBITOR SUBEROYLANILIDE HYDROXAMIC ACID TUMOR-SUPPRESSOR GENE HORMONE RECEPTOR GENE REGULATES E-CADHERIN PHASE-I MESENCHYMAL TRANSITIONS SP1 SITES TRANSCRIPTION SNAIL Involvement of HDAC1 in E-cadherin expression in prostate cancer cells; its implication for cell motility and invasion Article 10.1016/j.bbrc.2010.12.081 1 BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, v.404, no.4, pp.915 - 921 BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 404 4 915 921 scie scopus 000287173100005 2-s2.0-79151482937 Biochemistry & Molecular Biology Biophysics Biochemistry & Molecular Biology Biophysics Article HISTONE DEACETYLASE INHIBITOR SUBEROYLANILIDE HYDROXAMIC ACID TUMOR-SUPPRESSOR GENE HORMONE RECEPTOR GENE REGULATES E-CADHERIN PHASE-I MESENCHYMAL TRANSITIONS SP1 SITES TRANSCRIPTION SNAIL Invasion Motility E-cadherin Histone deacetylase inhibitors