Kim, Byoung Chan Zhao, Xueyan Ahn, Hye-Kyung Kim, Jae Hyun Lee, Hye-Jin Kim, Kyung Woo Nair, Sujith Hsiao, Erik Jia, Hongfei Oh, Min-Kyu Sang, Byoung In Kim, Beom-Soo Kim, Seong H. Kwon, Yongchai Ha, Su Gu, Man Bock Wang, Ping Kim, Jungbae 2024-01-20T17:35:11Z 2024-01-20T17:35:11Z 2021-09-02 2011-01-15 0956-5663 https://pubs.kist.re.kr/handle/201004/130727 This paper describes highly stable enzyme precipitate coatings (EPCs) on electrospun polymer nanofibers and carbon nanotubes (CNTs), and their potential applications in the development of highly sensitive biosensors and high-powered biofuel cells. EPCs of glucose oxidase (GOx) were prepared by precipitating GOx molecules in the presence of ammonium sulfate, then cross-linking the precipitated GOx aggregates on covalently attached enzyme molecules on the surface of nanomaterials. EPCs-GOx not only improved enzyme loading, but also retained high enzyme stability. For example, EPC-GOx on CNTs showed a 50 times higher activity per unit weight of CNTs than the conventional approach of covalent attachment, and its initial activity was maintained with negligible loss for 200 days. EPC-GOx on CNTs was entrapped by Nafion to prepare enzyme electrodes for glucose sensors and biofuel cells. The EPC-GOx electrode showed a higher sensitivity and a lower detection limit than an electrode prepared with covalently attached GOx (CA-GOx). The CA-GOx electrode showed an 80% drop in sensitivity after thermal treatment at 50 degrees C for 4 h, while the EPC-GOx electrode maintained its high sensitivity with negligible decrease under the same conditions. The use of EPC-GOx as the anode of a biofuel cell improved the power density, which was also stable even after thermal treatment of the enzyme anode at 50 degrees C. The excellent stability of the EPC-GOx electrode together with its high current output create new potential for the practical applications of enzyme-based glucose sensors and biofuel cells. (C) 2010 Elsevier B.V. All rights reserved. English ELSEVIER ADVANCED TECHNOLOGY CARBON NANOTUBES BIOFUEL CELLS GLUCOSE-OXIDASE HYBRID SYSTEMS IMMOBILIZATION NANOPARTICLE BIOSENSORS STABILIZATION BIOCATALYSTS PERFORMANCE Highly stable enzyme precipitate coatings and their electrochemical applications Article 10.1016/j.bios.2010.08.068 1 BIOSENSORS & BIOELECTRONICS, v.26, no.5, pp.1980 - 1986 BIOSENSORS & BIOELECTRONICS 26 5 1980 1986 scie scopus 000286904400031 2-s2.0-78650595283 Biophysics Biotechnology & Applied Microbiology Chemistry, Analytical Electrochemistry Nanoscience & Nanotechnology Biophysics Biotechnology & Applied Microbiology Chemistry Electrochemistry Science & Technology - Other Topics Article CARBON NANOTUBES BIOFUEL CELLS GLUCOSE-OXIDASE HYBRID SYSTEMS IMMOBILIZATION NANOPARTICLE BIOSENSORS STABILIZATION BIOCATALYSTS PERFORMANCE Enzyme stabilization Enzyme precipitate coatings Electrospun polymer nanofibers Carbon nanotubes Biosensors Biofuel cells