Kim, Jeong Kyu Jung, Kwang Hwa Noh, Ji Heon Eun, Jung Woo Bae, Hyun Jin Xie, Hong Jian Jang, Ja-June Ryu, Jae Chun Park, Won Sang Lee, Jung Young Nam, Suk Woo 2024-01-20T18:00:20Z 2024-01-20T18:00:20Z 2021-09-02 2011-01-15 0041-008X https://pubs.kist.re.kr/handle/201004/130729 In a previous report we demonstrated that the transcriptomic response of liver tissue was specific to toxicants, and a characteristic molecular signature could be used as an early prognostic biomarker in rats. It is necessary to determine the transcriptomic response to toxicants in peripheral blood for application to the human system. Volatile organic compounds (VOCs) comprise a major group of pollutants which significantly affect the chemistry of the atmosphere and human health. In this study we identified and validated the specific molecular signatures of toxicants in rat whole blood as early predictors of environmental toxicants. VOCs (dichloromethane, ethylbenzene, and trichloroethylene) were administered to 11-week-old SD male rats after 48 h of exposure, peripheral whole blood was subjected to expression profiling analysis. Unsupervised gene expression analysis resulted in a characteristic molecular signature for each toxicant. and supervised analysis identified 1,217 outlier genes as distinct molecular signatures discerning VOC exposure from healthy controls. Further analysis of multi-classification suggested 337 genes as early detective molecular markers for three VOCs with 100% accuracy. A large-scale gene expression analysis of a different VOC exposure animal model suggested that characteristic expression profiles exist in blood cells and multi-classification of this VOC-specific molecular signature can discriminate each toxicant at an early exposure time. This blood expression signature can thus be used as discernable surrogate marker for detection of biological responses to VOC exposure in an environment. (C) 2010 Elsevier Inc. All rights reserved. English ACADEMIC PRESS INC ELSEVIER SCIENCE HEPATOCELLULAR-CARCINOMA EXPRESSION TOXICOGENOMICS MECHANISMS MICROARRAY TOXICANTS PROFILES TOXICITY EXPOSURE SOLVENTS Identification of characteristic molecular signature for volatile organic compounds in peripheral blood of rat Article 10.1016/j.taap.2010.10.009 1 TOXICOLOGY AND APPLIED PHARMACOLOGY, v.250, no.2, pp.162 - 169 TOXICOLOGY AND APPLIED PHARMACOLOGY 250 2 162 169 scie scopus 000286128600009 2-s2.0-78650553501 Pharmacology & Pharmacy Toxicology Pharmacology & Pharmacy Toxicology Article HEPATOCELLULAR-CARCINOMA EXPRESSION TOXICOGENOMICS MECHANISMS MICROARRAY TOXICANTS PROFILES TOXICITY EXPOSURE SOLVENTS Volatile organic compound Dichloromethane Ethylbenzene Trichloroethylene Molecular signature Detection