Jung, Kwang Hwa Noh, Ji Heon Eun, Jung Woo Kim, Jeong Kyu Bae, Hyun Jin Xie, Hongjian Jang, Ja-June Ryu, Jae Chun Park, Won Sang Lee, Jung Young Nam, Suk Woo 2024-01-20T18:00:36Z 2024-01-20T18:00:36Z 2021-09-02 2011-01-01 0013-936X https://pubs.kist.re.kr/handle/201004/130740 Whole blood is one of the most easily accessible biofluids, and circulating leukocytes would include informative transcripts as a first line of immune defense for many disease processes. To demonstrate that transcriptomic responses of circulating blood cells reflect the exposure to environmental toxicants and the characteristic molecular signatures can discriminate and predict the type of toxicant at an early exposure time, we identified and validated characteristic gene expression profiles of rat whole blood after exposure to polycyclic aromatic hydrocarbons (PAHs). At an early exposure time point, conventional toxicological analysis including changes in the body and organ weight, histopathological examination, and blood biochemical analysis did not reflect any toxicant stresses. However, unsupervised gene expression analysis of blood cells resulted in a characteristic molecular signature for each toxicant Further analysis of multiclassification suggested 220 genes as early detective and surrogate markers for predicting each PAH with 100% accuracy. These findings suggest that the blood expression signature could be used as a predictable and discernible surrogate marker for detection and prediction of PAHs, and the use of these molecular markers may be more widely implemented in combination with more traditional techniques for assessment and prediction of toxicity exposure to PAHs from an environmental aspect English American Chemical Society JUNCTIONAL INTERCELLULAR COMMUNICATION DNA-ADDUCTS HEPATOCELLULAR-CARCINOMA CHEMICAL CARCINOGENS EXPRESSION PROFILES CELL-PROLIFERATION EPITHELIAL-CELLS MECHANISMS MICROARRAY TOXICITY Molecular Signature for Early Detection and Prediction of Polycyclic Aromatic Hydrocarbons in Peripheral Blood Article 10.1021/es101840s 1 Environmental Science & Technology, v.45, no.1, pp.300 - 306 Environmental Science & Technology 45 1 300 306 scie scopus 000285679300046 2-s2.0-78650710707 Engineering, Environmental Environmental Sciences Engineering Environmental Sciences & Ecology Article JUNCTIONAL INTERCELLULAR COMMUNICATION DNA-ADDUCTS HEPATOCELLULAR-CARCINOMA CHEMICAL CARCINOGENS EXPRESSION PROFILES CELL-PROLIFERATION EPITHELIAL-CELLS MECHANISMS MICROARRAY TOXICITY