Choi, Bo Gyu Park, Min Hee Cho, So-Hye Joo, Min Kyung Oh, Hye Jin Kim, Eun Hye Park, Kwideok Han, Dong Keun Jeong, Byeongmoon 2024-01-20T18:00:46Z 2024-01-20T18:00:46Z 2021-09-05 2011-01 1744-683X https://pubs.kist.re.kr/handle/201004/130748 Three dimensional (3D) cell culturing in an artificial matrix needs understanding of the dynamic microenvironments of the extracellular matrix and the cells. In this paper, we investigated a thermal gelling polyalanine-poloxamer-polyalanine (PA-PLX-PA) aqueous solution for chondrocyte 3D culture, focusing on the initial concentration of the polymer aqueous solution. As the polymer concentration increased from 7.0 wt. % to 10.0 wt. % and 15.0 wt. %, moduli of the in situ formed gels at 37 degrees C were increased from 350-380 Pa to 2100-2300 Pa and 5300-5700 Pa, respectively. In addition, the population and thickness of the nanofibers in the gel were increased. Chondrocytes kept their spherical phenotypes in the 3D environment of the in situ formed PA-PLX-PA hydrogel. They showed excellent cell viability, increased production of sGAG and type II collagen in PA-PLX-PA gel prepared from initial polymer concentration of 7.0 wt. % and 10.0 wt. %, emphasizing the significance of the micromechanical environments for the 3D cell culture. English ROYAL SOC CHEMISTRY INJECTABLE DELIVERY SECONDARY STRUCTURE CELL HYDROGELS COPOLYMERS CARTILAGE MATRIX POLYPEPTIDE INTERFACE DESIGN Thermal gelling polyalanine-poloxamine-polyalanine aqueous solution for chondrocytes 3D culture: Initial concentration effect Article 10.1039/c0sm00611d 1 SOFT MATTER, v.7, no.2, pp.456 - 462 SOFT MATTER 7 2 456 462 scie scopus 000286110900019 2-s2.0-78651358116 Chemistry, Physical Materials Science, Multidisciplinary Physics, Multidisciplinary Polymer Science Chemistry Materials Science Physics Polymer Science Article INJECTABLE DELIVERY SECONDARY STRUCTURE CELL HYDROGELS COPOLYMERS CARTILAGE MATRIX POLYPEPTIDE INTERFACE DESIGN Thermal gelling PA-PLX-PA concentration effect