Lee, Mina Kim, Dongwook Shin, Hee-Sup Sung, Ho-Geun Choi, Jee Hyun 2024-01-20T18:00:50Z 2024-01-20T18:00:50Z 2021-09-05 2011-01 1940-087X https://pubs.kist.re.kr/handle/201004/130751 Electroencephalogram (EEG) indicates the averaged electrical activity of the neuronal populations on a large-scale level. It is widely utilized as a noninvasive brain monitoring tool in cognitive neuroscience as well as a diagnostic tool for epilepsy and sleep disorders in neurology. However, the underlying mechanism of EEG rhythm generation is still under the veil. Recently introduced polyimide-based microelectrode (PBM-array) for high resolution mouse EEG(1) is one of the trials to answer the neurophysiological questions on EEG signals based on a rich genetic resource that the mouse model contains for the analysis of complex EEG generation process. This application of nanofabricated PBM-array to mouse skull is an efficient tool for collecting large-scale brain activity of transgenic mice and accommodates to identify the neural correlates to certain EEG rhythms in conjunction with behavior. However its ultra-thin thickness and bifurcated structure cause a trouble in handling and implantation of PBM-array. In the presented video, the preparation and surgery steps for the implantation of PBM-array on a mouse skull are described step by step. Handling and surgery tips to help researchers succeed in implantation are also provided. English MYJoVE Corporation High-density EEG Recordings of the Freely Moving Mice using Polyimide-based Microelectrode Article 10.3791/2562 1 Journal of Visualized Experiments, v.-, no.47 Journal of Visualized Experiments - 47 N scopus 000209212800050 2-s2.0-80355125532 Multidisciplinary Sciences Science & Technology - Other Topics Article Neuroscience Issue 47 Electroencephalography (EEG) Mouse Microelectrode Brain Imaging