Zhu, Lei Xie, Jin Swierczewska, Magdalena Zhang, Fan Quan, Qimeng Ma, Ying Fang, Xuexun Kim, Kwangmeyung Lee, Seulki Chen, Xiaoyuan 2024-01-20T18:01:05Z 2024-01-20T18:01:05Z 2021-09-05 2011-01 1838-7640 https://pubs.kist.re.kr/handle/201004/130763 We demonstrate the first true real-time in vivo video imaging of extracellular protease expression using an ultrafast-acting and extended-use activatable probe. This simple, one-step technique is capable of boosting fluorescent signals upon target protease cleavage as early as 30 minutes from injection in a small animal model and is able to sustain the strong fluorescent signal up to 24 hours. Using this method, we video imaged the expression and inhibition of matrix metalloproteinases (MMPs) in a tumor-bearing mouse model. The current platform can be universally applied to any target protease of interest with a known peptide substrate and is adaptable to a wide range of real-time imaging applications with high throughputs such as for in vivo drug screening, examinations of the therapeutic efficacy of drugs, and monitoring of disease onset and development in animal models. English IVYSPRING INT PUBL Real-Time Video Imaging of Protease Expression In Vivo Article 10.7150/thno/v01p0018 1 THERANOSTICS, v.1, pp.18 - 27 THERANOSTICS 1 18 27 N other 000299121000003 Medicine, Research & Experimental Research & Experimental Medicine Article CANCER PROBES matrix metalloproteinases (MMPs) activatable probes near-infrared fluorescence imaging real-time in vivo video imaging peptide substrate PEGylation